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Yazdani Ahmadabadi, Hossein

University of British Columbia

Long-term prevention of implant-associated infection (IAI) remains as a challenge. Despite advances in the development of antibiofilm coatings, current coating technologies fail to provide long-term prevention of IAIs. One of the most widely studied antimicrobial agents for generation of release-killing coatings is silver. However, current silver-based antibiofilm coatings suffer from major limitations that need to be addressed for widespread use in medical devices/implants. This thesis explores a new generation of silver-based antibiofilm coatings with long-term activity, high biocompatibility, and adaptability to medical devices and materials with diverse surface chemistry. In Chapter 2, the development of film-forming silver nanoparticles for generation of biocompatible durable long-acting coatings with zero bacterial colonization (for 28 days) is reported. The combination of two different molecular weight polymers with catechol results in effective incorporation of silver nanoparticles into a lubricious coating via a simple coating process. The optimal coating showed excellent broad-spectrum activity against eight different bacterial strains including difficult-to-kill and stone-forming bacteria. In Chapter 3, the development of a robust antibiofilm coating with long-term activity and high adaptability to virtually any arbitrary object was reported. The coating described in Chapter 2 was utilized in combination with a water-miscible organic solvent to generate such coatings. The optimal coating fully suppressed bacterial growth in solution and on a surface over the long-term irrespective of the bacterial strain tested. The coating resulted in sustained release of silver over the long-term (28 days). The coating retained its excellent antibiofilm performance following different challenging tests. Antibiofilm efficacy and excellent biocompatibility of the coating were demonstrated in vivo using mice and rat models. In Chapter 4, the generation of film-forming supramolecular assemblies utilizing catechol chemistry in combination with a low molecular weight amphiphilic polymer is discussed. The supramolecular assemblies resulted in relatively thick coatings with great surface coverage and high hydrophilicity. The optimal catechol/polymer coating was highly effective in inhibition of biofilm formation over the long-term. Based on this work, three sets of novel antibiofilm coatings with long-term activity were developed utilizing catechol chemistry in combination with silver and hydrophilic polymers.

Text

2024-09-30

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/80765

Chemistry

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/