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Hu, Jinhong

University of British Columbia

The bacterial type III secretion system, or injectisome, is a syringe-shaped nanomachine essential for the virulence of many pathogenic Gram-negative bacteria. A major functional subcomplex of the injectisome, the needle complex, is a 3.5MDa complex formed by more than ten unique proteins. The needle complex forms a continuous channel spanning both the inner and outer membranes of Gram-negative pathogens, created by three highly oligomerized inner and outer membrane hollow rings and a polymerized helical needle filament. The effector proteins secreted through this channel, which vary amongst different bacterial species, are essential for subsequent pathogenicity. Thus, structural studies of this complex can provide important atomic level information for understanding complex assembly and function of the injectisome as well as potentially development of new antivirulence drugs or vaccines to combat infections in susceptible human, animal and plant hosts pathogens. The first high-resolution needle complex structures determined by cryogenic electron microscopy (cryo-EM) here shows the atomic details of the inner and outer membrane protein complex and the needle filaments. The outer membrane component of the needle complex belongs to the secretin family, a giant necessarily gated pore common and essential to other bacterial secretion systems but which had remained largely uncharacterized at the atomic level until recent work including major contributions as outlined in this thesis. Further, the structures of the dual nested rings that form the major inner membrane structural component of the needle complex shows remarkable similarity regardless of the assembly stages, inferring a highly stable foundation for the other components of the system to pack and function within. The snapshots of multiple needle complexes at different assembly stages revealed multiple new structures, the dynamics and the sequence of the assembly. The structural information also answered several long-standing additional questions, such as the mystery of the apparent symmetry mismatch between the inner and outer membrane complex of prior structures, and the fold, span and functional role of the historically named “inner rod” protein which this thesis works shows is not a rod but an adaptor to set the needle helicity and anchor it to the stable inner membrane platform.

Text

2021-12-20

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/80481

Biochemistry and Molecular Biology

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/