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UBC Theses and Dissertations

The impact of genetic variation in ABCA1 on cholesterol metabolism, atherosclerosis and diabetes Brunham, Liam Robert 2007

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E IMCOGEC AOIAA I OCSMESMAESCSIS A ASAM  UAMScwi so e Ui oMaA ESIS SUIEIIAIME OE QUIMES E GE OCOMCOISOY A COMECIiE CUY OGA SES(MecaGecsE UI OISCOMA am uamAace A-i cassee ae sumi Amem1 (AA1meas emaway cseom c ces a e co e -mi sie ogesis ogi oi( icsImaAA1 ciey sus iieseasecc w  ocs ceucsaccumuoa iasesk aescsisMoa1 co as  escie AA1 geWe aemeue w  y occui a eiemuo iAA1 imci icsa ia e oio a i sysmsWe aemecoe scic gec as iAA 1 wi es ies cayi eseasa useaeo aac ecwcscic as iAA1wou imci o We ee e s ossue-scic gec ooAA1 imice  s i  iogesisaescsis a gcosemesmWe u aiesAA1 is aima si oogesis aaacooiesAA1 ises  ic AA1wcisa masi oogesiswas sw sigca co  suscei aescsisywe sw aAA1 ays auusc iceoa iuseco ese ses  co ouuei oeimcogec aoiAA1 oe ogicaa ogicaocessesa  iieasc oAA 1 oiscic ce siie oCoesAac ^e oCoes ^ iiisoes ^ sogus ^ isoAao  ^ iiAckwemes ^Co-AuoSme ^ 1 Ce1 - Ico ^ 11 Csiaa sease ^ 11 Emiogy a ogesis oAescsis ^ 31 Aescsis as a e Mec Syme ^ 51 Csa oiMesm ^ 71 Mesm a Gecs  ^ 91 AA1 a CsE ^ 11 AA1 ysiogy ^ 11 EemeaAacs  Uei e  oGec aoiAA1 iCsMesmAescsis a Gcose meossis ^ 1 Oecs a eses ^ 1 e s ^  Ce- ao oa Ge ^ 351 Ico ^ 35 IicaooGes agu Cs ^ 33 A Ge Suami ^ 37 MocuEooAA1 ^ 395 Muo iAA1 ^  ec ImcoMuo iAA1 ^ 7 e  o AA1 as ie Geo ^  Commoas iAA1 ^ 59 oc Ac oAA 1 ^ 531 Cosio ^ 5711 e s ^ 53Ce3 - Accu ecooe oSigcae oSi comosms a Muo ie  AA1  Ge ^ ^3^ Ico ^ ^3^ sus ^ 7^3 ^ ecoooeecoAA1 muo a cSs ^ 7^3 ^ oAssessme oAA1 as  ^ 71^3 ^ Assessme o AA1 as iieie geo77^3 ^ Comsowi e ^ 7^3^ scussio ^ 79^3^ Meo ^ ^3 ^  Se ^ ^3 ^ CauooSuosioScic Eo CoeaoScos iii^3 ^ GeooSe Ce s ^ ^3 ^ Wes oi a - ^ 9^3 ^ CsE ^ 9^3^ e s ^ 9 Ce- IesAA1 cy Cos  ogesis I ^ 95^1 ^ Ico ^ 95^ ^ sus ^ 97^3 ^ scussio ^  11^ ^ Meo ^  1^1 ^ Ama ^  1^ ^ Soue Asis ^  1^3 ^ Wes Asis a aime  ^ 1^ ^ Immuuosee ^  1^5 ^ asma a ssue asis ^  1^ ^ Eecy ses ^  1^7 ^ Csaoo ^  1^ ^ Meseec m coeco ^  1^9 ^ Sscaasis ^  1^5 ^ e s ^  15 Ce5 - IesScic IucooAA1 Essiowi a Agosises asma Cs ^  131^5^ Ico ^  131^5 ^ sus ^  13^5^ scussio ^  13^5^ Meo ^  13^5 ^ e s ^  1 Ce- Coooc AA1  Suscei  Aescsis 1^1 ^ Ico ^  1^ ^ sus ^  1^1^ GeooAe  ;Aa -1-1- ^ 1^^ asma  ^  1^3 ^ Suscei  Aescsis ^  1^3 ^ scussio ^  1^ ^ Meo ^  151^1 ^ Ama ^  151^ ^ Wes Asis a aime  ^ 15^3 ^ asma  ^  15^^ Aescsis quaicao ^  15^5 ^ e s ^  157 Ce7 - e  oce AA1 iGcose meossisIuSecoa se  iao eae ^  157^7^ Ico ^  157^7^ sus ^  15^7 ^ Aaessioa oimu is ^  15^7 ^ Gcose ioec iuseco a aececsmeossis iAa -- mice^  1i^7 ^ ce AA1 ie se  iao eae ^  177^ scussio ^  1737^Meo ^  177^7 ^ Ama ^  177^7 ^ ame  Wes oi a immuouscee ^  179^7 ^ Sscaasis ^  17^e s ^  1 Ce- Cosio ^  11 ^ AA1 gecs ^  1 ^ogesis  ^  13 ^ AA1 a aescsis ^  1 AA1 a gcose mesm ^  15 ^ co ^  19 ^ Summa ^ 1917 ^e s ^  19Aei1 Co oU AmaCa Commiee Ceicas ^  19soese 1 maseases aec mesm ^  1e 1 Ges ioeimesm wc mamuo  ee ^  1e 1 A ae associawi seases ima ^ 3e Muo iAA1 ^ e 3 e es associawi gous AA1 muo ^ e symous Ss i AA1 ^ 5e 5 AA1 g Ss ^ 5e 3 Csees 3 ces acwi AA1 as asuSEC a eeco oe oimcoese as ^ 73e 3suSEC a escos AA1 as scia co oiia wi w csom e geo Macageees a as e Coea (15  ^ 77e 1 asma a aoies AA1 iesscic kck-oumice coumi a sa cw e ^  1e 1 asma a AoiCoeo ^  1e 7 asma mec me i Abl +1+ Abl --  mice ^  1sogusgu 1 Acw ogiaic sc eemes iwccse oi iucescusio semi maaescsis ^ gu 1 ways omesm ^  1gu 1 Ay Waos ysiogicaawi ^ gu 1 Kaa ic"Ma a Maus" ^ gu 1 age Scic Eoo AAI  ^ gu caooMuo i AA] ^ gu 3 ComsoosuSEC scos  AAI  cSsmuocescas ia co oiia wi w C om e geo a a om sioow equey aes ^  71gu 3 CoeaooAA1 ami acisio11 ioi aoeecomuoais si ^ 75gu 3 Coeoocsees wi e o oa oimie ( ous  AA1  muo a Ss ^ 7gu 3 Gicaeseaooe eo oeemousemaa cmee AA 1 oi ^ gu 1 GeooAA1 iesscic kckoumice  (Ab/ 1  ^ 9gu EssiooAA1 imouse ies ^  1gu 3 Asis oasma oi  soiquicomagy (^ 1gu ssue-scic coo oAA 1  asma cs 1gu 5 Iescsa imice cki iesAA1 ^ 1gu ssue cs a ge essio  ^  1gu 7 Cssecoom ima eecys ^  11gu Asis om oi i Abl  +  a  Abl -`  mice ^  11gu 5 asma cs ico(/ (--a ies(-i/-iscic AA1 kck-oumice ase ^  133gu 5 asma   a aeeae wi GW39 ^ 13gu 5 Iesa c mA a oi imice eawi GW39oc ^  135gu 1 AA1 oiessioia macages ^  1gu soiquiComagy o oasma cs ^  1gu 3 Suscei  aescsis imice cki c AA ^ 1gu 7 Gcose meossis iAagkck-oumice  (Abl-/- a Aaessioiis ^  159gu 7 Geoomice cki Aaices (Aa- ^  1gu 7 Gcose meossis iAace scic kck-oumice ^  1gu 7 ce mass imice cki ce Aa ^  1gu 7 Aee oce Aasus iaecsmeossis iisois ^  1gu 7 Iusecois imiiisois cki ce Aa ^  1gu 7 ce Aais esseiae se  sig eae ^  17gu 1 Uaways omesm ^  1isoAaoAA 1^ A-i cassee aesuami A mem1AG1 ^ A-i cassee aesuami G mem1AG5/^ A-i cassee aesuami G mem 5 a ACA^ AcyCoAsAcyseA ^ aoiCA^ co ae seaseCE^ csesCE ^ csesaoiE^ eoeaseA ^ ee y aci ^ gi oi ^ c seGC ^ G-CoA cseGCS ^ G-CoA syse ^ w i oi ^ w i oiceo ^ oise ^ ceoC 1 1 ^ ema-ck Cke 1^ oso ^osoaoi ^ e csaiiS ^ si co mosmS1 ^ Scaeceocss  1suSEC ^ suosioscic eo coeaosco ^  as^ iesease ^ igce ^ igce coi ^  w i oiiAckwemesis wo is ca my mi a osWiouyou a suo ois wou  a sigcae  mea you  my s gi me eeimyse  ue my eamsSciaas  SK maki ose eamscome 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(gu 7a is i sugges aeimie iiusecoiAa-cie is es weam oiesei a secoa siggeo e 13-ceIucoe was iaseiis cki Aa(gu 7 as we as iw a om  Aa --  mice(gu 7 w sc iumA  we sigca ae(gu 7 cois wi a cie ecysis oiucoai sec gsEmiooe mics ogcose-smuiuseco siooisois moaa ciase gcose-smuiuase imice cki 13-ce Aa(gu 71a5- +/+ 33cCI -- 11g ;51- c a3--  I E 1C- 1 - 5- 5Gcose 17 mMKCI mM ^ 17 mM mM+/+ --Aa ge ^Aa ge ^ 1^ CI  +/+C  c 3-1E ac cm o EE   fIA1- CCC a ^ +/+ --^ In ^ In15=Abl+ 1=Ab1 --a1 -o5C17-   Ab 1+1+- Ab1 --1515c 1-15c a 7   5e  5e^ 1^  ^ 3^  ^ ^  ^  ^ ase mM gcose ^ AUC^ AUCme (mi  gu 7 Iusecois imiiisois cki ^ Aa 1(aiusecoomisoisIs we cuuoig io smuoie coio iica1 u eses o om 3 se eemes eaccois oois om 3 ama gea es a esseas e oiscoe   sasecowcis aiaseas 1( isiucoe(cac iucoe( mA essioo In  a  In  ges iis isoom  Abl --  mice a co(eIssio ( aa uee cue om issioeeme(g (uea oum io ( si oas seco omAbr   (ga  Abl --  ( mice1gu ( ( a (3 gu(c (ea ( 1iS sEcysis oiucoai gs is oug  occuascic micmai oe asma memaa eooasma mema css ee eae gcose-smuiuseco13 We emiceucs iseco oas om  Aa -  -  mice usi ecso  Is om  Aa -  -  mice sayea maeiase ie1iei osi (gu 7 cois wi ouquai asis oiscscoesi aea  scicay eaeaeasma mema oisces om  Ab1 --  miceese  ise e ssiac Aa1-meaeom 13-ces a  csaccumuoae asma mema wcis associawi imiiuseco7ll AA 1  th tzntntiaos (sucas sig a agos oe caoceoA a a wi useie eae o A is kwac AA1 imacages a e ceo((1We u asig eae sigca iaseAa1 essioie aece I-1 (gu 7a We e esiasig-meaiucoo13-ce AA1 cou i co  e ciaeecoesecomus o13-ce oa gcose meossis sis esis we  Abl --  a comice a ge(3 weiga oicomowi 3mg/kg/y sig  egimesueisigca weig gaiaeweeksweig esigcaeeges ia gu( g   g ocw g   gog  g   g ogus sig Abl +1+ Abl -- sc Gcose e s we oeio a weeks aeiaoogege sueisigca imigcosee iwi micea sig sgcose e (gu 7ge ao woegcose e i Abl --  micesig eecogcose e iese ama (gu 7c e aa ue17e gcose e  cue (AUCi   was iasei Ab1 +I+e a e  sigca e om aocw-miceowi sig (gu 7Icos e  AUCi   Ab1 --  mice wassigca iase gea imo ise sig (gu 7s iu we sigca iasei Ab1 + mice a gea we e se   sig (gu  7Ab1 -13I- miceswe ce is iu ise  eiegeosig (gu 7e Iusei was imo a simiee iges ise  sig (gu 7iica ae ee igcose e oeeee Ab1 +I+ Abl --  mice cou ei e eec osig oiusei171b1E 353o 151aGaCo ^ si   -- +1+ cw+/+  ^ 35 si E E 37; o 15(5 -o--Cw si1 3 5 71 91Mis aeigcose ieco1 3 5 7 91M s aei  gcose iecoAbl  ge ^ Ab1  ge_ =Cw ^ 1I= + si ^ E 19E E1 o 5(7 1-111ao E  e-EAb1  ge1  3 5 7Mis aei iuiecob  1 ge^  ^  Is  I^[  E^ // [^ E^ I  sig31u--+7- ^1+ --gu 7  ce Aa1 is esseiae se  sig eae (a Wes oAa1 iI-1 ces owi oig cuu wi 1  sig oco  (  gcosee s i Aa l+ cei a cw e a aeweeks ogogsig e1gu  (c gcose e s i Aa -  -  cei acw e a aeweeks ogogsig e1guSscacomsooI cues i (  a  (cis  ameasus A wiumaKeu ss  (  Aa uee igcose e cue (AUCie micesci (  a  (c (e s asma iucoeo e mice sci (  a(c  gu  (  iue s i Aa +1+ Aa ^ mi cegsig egu  ( i isee cs  (  a igce- (iiis isoom  Aa+1+ Aa -  mice aeweeks ogogsig e we i gas-quicomagy aeymac assay oois om 3 mice gu a eo ese saaooieassay ao   sigcasig wee ee cscoeoiis isoom wimice (gu 7g ee cs we giis isoom  Ab1 --^ mice a we ue sig (gu 7g sugges aai weiscscoe may co  e i  s  sigiese miceysig weis coeoequi iges omice (gu 7177^ scussioWe  cumeea  AA1 imea csmeossis aiusecoiac -cesAa1 is gy essei-ces a aeeoce Aa1 sus iaccumuooceucs maecoiiusecoi a ogssi imie igcose ee ciiusecois e  a coice masssugges aAA1 is ioeiise oimaiee oce massIusecoissigca ceiis isoom mice cki 13-ce Aa1swi aceucsmeossis ays a cciao iisywe swa(3-ce AA1 cos  e ciaeecoiaos ogcose esugges ascic ueguoo(3-ce AA1 may  auseeuc aac imo ce oi asw  ocseque co-occuwi  as ie coe oe mec syme(15 we a  csic sease oiss ecay esis oiceoiicue w ioi( ceoa oia esseimouse is(1a aegec oi sucas a  iuce aoc a iisois a aece s(11aeecocke gi oi((1e cue ses a e  oukwe  sw a scic cice csmeossis associawi imi-ce o173 a commoaoae  AA1  cus is aima i ocs ie geo19; is ises e ssi aaeo iAA1 may  aima ui mecsm ueyi  e wcsa imice oiiia wi a 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ci Aa --  issis ae oiuecysisweam ogcose sei omesmCeucs  esw mo ecysis ia e oueoc ce s(- Ousus imica AA1 a ceucsmeossis iiuecysis iScicaywe sw wi si ais cki 3-ce Aa1  accumuoocs ke ae asmamemaa ais is associawi imiiuseco Imai  iiooAa1 imi oemeosc sysms ie ce aco  issoa imigcose e iese miceiaos (sucas sig a wi useie eae oasIaio eiiusei aco a cog ciaeec oce oima(a 3-ce mass a oiamamo( Cuu oisoac is ie esee oe 175og s esw weceucoe(3 a eae ceo e mocueo is Aa cao ceoioeigcose a mesm a ce eiao A iuces AA1essioa csea imacages(131 A-iuceacooAA1 eaes e mooceucsa es accumuoimacages(1 a mesaiaces(3We e esiaacooA wou ao a acooAA1 icesa aiseeccou co  e ciaeeco oce oa gcosemeossis isig eae ceis coe i  Abl+   a  Abl --  micea weisee cs iwi  Aa --  miceis wasassociawi sigca imogcose e iwi mice wi  eecosi ogcose e imice cie ce Aa1sigimoiue equay i gese i osig imo gcose e i Aa --  mice sugges ascic acooceAA1 a sueque cooiscscoe is aimamecsm  wcsig imos gcose eImai  iwee eecosig oAA1 quis ce iwas eamice cie  a maacioacoAA1say imigcose e a cece o geewiaccumuooua iis(33 e mec ciese mice is simi wwe oeei Aa --  micea ieea coois Aa mA17was oeei 13   micesugges amoooAa1  may ieie e iese miceIsummawe moa aAA1 ays a ccao imeacsmeossis icesa aaee oce AA1 a  ciusecoa imie ogcose esugges acsci may  aima com oc sease oissigwcacs AA1imos gcose e ie esee ieaee oce AA1sugges asagies wcscicay eceAA1 may  ciaimo ce oiw ai euesie eec o oaogesis7^ Meo7AnAa1 gkck-oumice we geus o OmaaoiGsea  Ab1  omice  eeous sc Ab1  cescic kck-oumice a co we ge cssi  Ab 1  omice oa miC571Sckgu wi g-(Ic5Mgmice (ckso9 Ama ceia sa  cwoge(ak 935wi owiou3/sig ( yie a se o3mg/kg/yseomeasuoiake as iica A ses we a eUi oisComa AmaCa Commiee7l d t Iagcose e s we oeousmice iecwi mg/g gcoseoogcose was measuwi a gcome(scaIu177e s we oeousmice iecwi 1 U/kg macom iu( isk Iusecoi was iiusmice iecwi 3 mg/g gcose o3 mg/g aiasma iuwasi ESA (CsCm Iusecoio was oeo-ckeis isoowi i-ccoagese iecoas eoussc3Is we cuuoig im meaaa is we iKs ecao 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aescsisw ooAA1 imacages iases suscei  aescsis wiouci asma (1Icos mice wi gicooAA1   iasesuscei 1aescsis eoaegec gec ckgus (1misce oe ss aeeciase iaescsis imaO eoese sus is aAA1 imacages ays aima ai-aegec   ei accumuoa ii am ce ao essigca co  asma csIcos mice wi gooAA1cause oeicoics  oe ce isuscei  aescsis com cowe ese sus aosuggesas oe  oAA1 iaescsis iscic ssues may yieiigs aa oaie  si gkck-oumoc AA1 is emaco asma csa a quesowas e ecoooc AA1  aeseois susceiWe moaamice wi scic ooAA 1 oe aE-cieckgu  iasesuscei  aescsisa accumu csieiaoasis su s imicao oucoeiooe csa i ge ics ie  asma cs a ce Eieesee oe-c AA1is sus ia miscaci  mo eesscsom essues a a sueque iase iaescsisese sus a ioa om a eucs cMacage AA1 is wicoiaai-aegec aacogicae is cay cicay icu scicay e c AA1 icosis  easy  e sucas  usi ge a is ecoiawee eecoaescsis wou 1cia Ousus suggesac AA1 ue oi  oogesises ay aai-aegec  a cou e ese aac euc e ^AA1 a gcose mesmAA1 is esseima ssues ie  (17oy e a ies aea ima e ocooicsWis e ooAA 1 ioessues? We scoseius amice cki AA1  imigcoseea aAA1 oiis esseie is oeasugges aAA1 may ay a eous uecog iiso e i amice wiscic icooce AA1  ou gcose ioe a c iusecoi a io iassociaowi aece csmeossisesisa w  AA1 ice o Mo oayese ses suggesacsmeossis may  aima i oce oWe moaace AA1 is quie ciaeec osigogcose esig is o oe moscommoy useugs ie eae o as wi o1miioescio ie USs iWsig is cog  ciaeec oce o e mecsmsueyi ese eec we we esis Ou swi a quime ceAA1 ie se  sig sugges a oe ciaeecoiscomu is  gu ceucsmeossis a AA1is ao sugges aeuc sagies ascicay ece AA1 may  ciaeec oceow ciumi e ga eec o oagesis15 ^  coSes o iesgaoa sugges ese sesyiwi  oies ee ousus oe eciooe ocoequees oAA 1 as oeges wi esisoassaysIousus wi AA 1 o gee oeges iwou suggesae eo-seaacwe emoyecou  ausemeoigemaki ge-e ecoOusus iica aiesAA 1 is aima soue oasma csise e quesoow iesAA1 may ay iaegesis cssi micewi iesscic ooAA1  o oe esissai oaescsis-suceie mice isu  ssie  aweis queso Iaio iwi  oiess wesuscei  aescsis is moe iesscic acooAA1e iaseaescsis imice cki c AA 1 sugges aockage oisscic sie way iases aegesisIwi  oiesi ses measu s oe csa iese micesucas usi macages (1o assessi e eiaose om ese mice  mo accui e imcoc ogesis osyiwi  ioa  ee ouses oAA1 oiiso s oiui a immea sugges  s oe ecise mecsm  wcAA1 acsmeossis iees iuseco Isu  ssie  gaiiig io isquesousi ce ogy mo oe eecoceucsaccumuooscicasc oiuecysisSecois ce imie ie aee oAA1 woe19 smia?  cssi  Abl -  mice  smic sai ousious ggcse gime isu  ssie  i wee e iese mice ca ecea woe e ge oceucsaccumuo ^ SummaWe emoye y occui a eieas oAA1 ia e omosysms ioe s e oois aeiysiogicaocesses a seaseogesisis s aoweus  ii w s oAA 1 iscic ssues iogesisceucsmeossisaescsis a ase sus oeseses  suo  e oosi  seases  gaiameaiigs ioogicaocesses as we as e use ossue-scic ge e ioe ssecesc oogesis wo ao moas w a si ge caee muiea myao isc ssuesai come a woe e mysousemeo1917 ^ e s1 egesoa Aoooies a miISiseeas Gasoiesa seaseMema ieaa SiseeMeSaueia1715-175 Ge a GckmaM197 ies secs scoigioi   Clnvt  -533 sG  sgaieC a GckmaM19ies secssecagi oi Ch 59359 a Sma19 csmo  oasma gioi Adv   17-515 aoGckmaM Weie Gea 19Eecoa ioomeseec m aoiI a gi oi  Clnvt  17 Oi useK Meia Quiao1993Cses im cmiccooom gi oiaeom asmaio iesm p   317-1737 Si Oi scoes Caso ao aQuiao19Coooasma oia oi  iesmcomsoo-ciwi meum-ciigce oiusio  h3-19 scoes Si Oi Caso  ccia aQuiao19Ogioiesm csicooom miaoo mucosasysis a oom asma h -19 Quiao ewiacki Sca  a a Si1979Ogiocsaeiiesmses ies wi aca p  -91 Imaimi i a g197 Ogia a oe A-Ia ai-caoi imeseec m o p   19111 aSa o CACEO IMESMe e a osmia Annl v M 5713-31 Sc k  Me  SceS WaooM Mae    o icoogesis Gv 131-31913 GoA Eia soscKok oks Si kes mi a   Smuoogesis  aacogicaAcooe ceoa  ocooeigce-c w i oiics n  Chtr 7-391 Sia eC Caso mes uyeCe ssaCy uc McMas   IaseAA1 ac ocagaiaescsis Clnvt  1135-15 Eck Sia Ye ea mes yM a eCMacage A-i Cassee aeAOssioIi Aescc sioogssioiw-i oiceoKckouMice Artl rb    -931 Aieo ees ussa yesey Coskgassaa aoIaseaescsis iimic mice wiicooAA1 imacages Artlhrb 3717 WeiC WaeE SuaA Kwok ssa Sia Yaa mes WioE   AA1 mA a oisioes ecmuie e s a  oguo  b Invt  73-31 a i iy Gck aG a  OssiooAoiA-I omos e a oCsomMacages  ces I Cln  1-3193Aei1 Co oU AmaCa Commiee Ceicase Ui oisComaAMACA CEICAdrOCO M  A-IESGAOCOUE C ^ MME M GOCE d Ctrn  p AA1 tvd Cpn d AthAMA MA^ --1e AmaCa Commiee s emia ae use oama e aee ogma  egiaassue ae ama ioewi  caiaccoae wi e iies coaiiCa oEemeaAma - A Gui Cais e CaaCouioAmaCaAoe U Commiee oAmaCa  o oWMiomCi c AmaCa CeM MacMageis ceica is o yeaom e a a oe is  ce ie eemeaocesAuaew is qui e CCAC a some giageiesA co ois ceica mus sayeiyouamaciOice oseaSeices a Aisao11 9Agmy a ou 1o7-5111  5319e Ui oisComaAMACA CEICAOCOM  A-IESGAOCOUE C ^ MME CMMOCOCOUE E Ctrn  p AA1 ttld n d tthAMA M3S  -- ^ A  -  - 15I AGEY t d Strdn  CYe AmaCa Commiee s emia ae use oama e aeemeaocoac couea  egiaassue ae amaioewi  caiaccoae wi e iies coaiiCa oEemeaAma - A Gui Cais e CaaCouioAmaCaAoe U Commiee oAmaCa  o oWMiomCi c AmaCa CeMs MacMage AmaCa Commieeis ceica is o yeaom e a s oa (wcis oe is  ce ie eemeaocesAuaew is qui eCCAC a some gi ageiesA co  ois ceica mus sayeiyouamaciOice oseaSeices a Aisao1Agmy a ou 1o7-5111  53195

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