UBC Faculty Research and Publications

Comparative genomics of resistance of spruce to the white pine weevil in British Columbia Ritland, Kermit; Verne, Sébastien; Jaquish, Barry; Ritland, Carol Sep 13, 2011

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ORAL PRESENTATION Open AccessComparative genomics of resistance of spruce tothe white pine weevil in British ColumbiaKermit Ritland1*, Sébastien Verne1, Barry Jaquish2, Carol Ritland1From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and DeliveryArraial d Ajuda, Bahia, Brazil. 26 June - 2 July 2011We present results of two large scale comparative stu-dies of the genomic basis of resistance of Interior spruceto the white pine weevil. Both volume growth, the mainobjective of the spruce breeding program in BritishColumbia, and white pine weevil resistance, are exam-ined. “Interior spruce” is a species complex involvingmainly Picea glauca (White spruce) but introgressedwith P. engelmannii (Englemann spruce), dependingupon locality. In the first study, we compared constitu-tive expression of 17825 genes between 20 resistant and20 susceptible trees to the weevil; 54 upregulated and137 downregulated genes were found in resistant pheno-types, with implications discussed in regard to volumegrowth. In particular, we will be surveying these genesfor SNPs that differ between these two classes of treesin the next year. In the second study, we developed a1536 Illumina SNP chip based upon candidate genes forweevil resistance. In a novel experimental design, weassayed 945 open-pollinated progeny of the PrinceGeorge breeding population (176 parents), and 654open-pollinated progeny of the Prince Rupert breedingpopulation (134 parents); parents were also genotyped.Within each family of 100 progeny, we identified thehighest ranked 3 progeny and the lowest ranked 3 pro-geny, based upon BC Ministry of Forests scores forvolume growth and resistance. These were genotypedand used in a novel test analogous to the transmissiondisequilibrium test to detect both SNP associations andQTLs linked to SNP markers. Discoveries about associa-tions and QTL are discussed, with the added cautionabout genotyping error. Both studies illustrate howoperational tree breeding populations can provide valu-able inferences about tree genomics.Author details1University of British Columbia, USA. 2Ministry of Forests, Lands and NaturalResource Operations, USA.Published: 13 September 2011doi:10.1186/1753-6561-5-S7-O10Cite this article as: Ritland et al.: Comparative genomics of resistance ofspruce to the white pine weevil in British Columbia. BMC Proceedings2011 5(Suppl 7):O10.Submit your next manuscript to BioMed Centraland take full advantage of: • Convenient online submission• Thorough peer review• No space constraints or color figure charges• Immediate publication on acceptance• Inclusion in PubMed, CAS, Scopus and Google Scholar• Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submit* Correspondence: kermit.ritland@ubc.ca1University of British Columbia, USAFull list of author information is available at the end of the articleRitland et al. BMC Proceedings 2011, 5(Suppl 7):O10http://www.biomedcentral.com/1753-6561/5/S7/O10© 2011 Ritland et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.


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