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Genetic determinants of cocaine-associated agranulocytosis Buxton, Jane A; Omura, John; Kuo, Margot; Ross, Colin; Tzemis, Despina; Purssell, Roy; Gardy, Jennifer; Carleton, Bruce Jun 13, 2015

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Buxton et al. BMC Res Notes  (2015) 8:240 DOI 10.1186/s13104-015-1219-4RESEARCH ARTICLEGenetic determinants of cocaine-associated agranulocytosisJane A Buxton1,2*, John Omura2, Margot Kuo1, Colin Ross3, Despina Tzemis1, Roy Purssell4, Jennifer Gardy1,2  and Bruce Carleton3Abstract Background: Drug-induced agranulocytosis is a recognized adverse drug event associated with serious infectious complications. Levamisole is an antihelminthic and immunomodulator withdrawn from North American markets in 2005 after reports of agranulocytosis. Previous studies of levamisole, suggest that the human leukocyte antigen (HLA)-B27 confers a genetic predisposition to this adverse drug event. Since 2009, emergency room visits due to agranulo-cytosis in individuals consuming levamisole-adulterated crack-cocaine have increased.Methods: We performed a case–control study using a genotyping assay and novel gene chip to test the association between cocaine-associated agranulocytosis (CAA) and HLA-B27 and to identify pharmacokinetic and pharmacody-namic gene variants associated with the phenotype.Results: Fifty-one CAA cases were identified through a provincial physician reporting system between 2008 and 2011. We examined eight of these cases and 26 matched controls. Genotyping revealed a significant associa-tion between HLA-B27 and CAA (odds ratio [OR] 9.2, 95% confidence interval [CI], 1.54–54.6). We also observed a similar association with the HLA-B27 tag single nucleotide polymorphism rs4349859 (OR 9.2, 95% CI 1.5–54.6) and rs13202464 (OR 6.7, 95% CI 1.1–41). Further associations were identified with variants in the ARBCC12 (OR 10.0, 95% CI 2.7–36.8) and CYP11A1 (OR 7.4, 95% CI 2.1–26.6) genes, while a novel protective association was observed with vari-ants in the ARDB1 gene (OR 0.06, 95% CI 0.007–0.46).Conclusions: We confirmed the association of HLA-B27 with CAA and identified additional susceptibility variants. Health care providers should inform people who are identified as having CAA that it is genetically determined and can recur with continued cocaine use. The severity of infections and subsequent hospitalization, and the risk of recur-rence may prompt health-promoting behaviour changes of the affected individuals. These genetic associations war-rant the attention of public health and knowledge translation efforts to highlight the implications for susceptibility to this severe adverse drug event.Keywords: Agranulocytosis, Levamisole, Cocaine, HLA-B27 antigen, Pharmacogenetics, Neutropenia© 2015 Buxton et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.BackgroundAgranulocytosis is a recognized adverse drug event char-acterized by a decrease in peripheral neutrophil count to less than 500/µL (0.5 × 109/L) [1], with affected individ-uals at increased risk of infection, sepsis, and death [2]. Multiple drugs have been implicated as potential triggers of agranulocytosis, including levamisole [3]. Originally formulated as a veterinary antihelminthic, levamisole was later used to treat autoimmune disorders including rheu-matoid arthritis (RA) and as adjuvant chemotherapy for colon cancer. Agranulocytosis became a well-recognized complication of levamisole therapy, occurring in 2.5–13% of exposed patients [4]. These findings eventually led to its voluntary withdrawal from the US market in 1999 [5] and the Canadian market in 2005 [6], although levami-sole continues to be used in veterinary medicine as an antihelminthic in the USA, Canada and South America [5, 6].Open Access*Correspondence:  jane.buxton@bccdc.ca 1 BC Center for Disease Control, 655 West 12th Avenue,  Vancouver, BC V5Z 4R4, CanadaFull list of author information is available at the end of the articlePage 2 of 6Buxton et al. BMC Res Notes  (2015) 8:240 In 2005, levamisole was first identified as a cocaine adulterant [7] and by July 2009 was found in as many as 69% of seized cocaine samples tested by the United States Drug Enforcement Administration [8]. Reports of agran-ulocytosis in individuals using levamisole-adulterated cocaine first emerged in 2009 from Alberta and British Columbia (BC), Canada [6, 9] and New Mexico, USA [8] with later reports from other parts of Canada and the United States demonstrating a spectrum of pathology including cutaneous vasculopathy [10–13]. Relapse is common with approximately half of patients experienc-ing more than one episode of agranulocytosis associated with return to cocaine use [6].The mechanism by which levamisole induces agranu-locytosis is thought to be immune-mediated. While no reported cases of levamisole-induced agranulocytosis could be identified from the period when the drug was used primarily as an antihelminthic agent, case reports emerged when its treatment profile broadened to include RA in the late 1970s and early 1980s [14, 15]. Stud-ies of affected RA patients demonstrated an association between human leukocyte antigen (HLA)-B27 positivity and levamisole toxicity [16–18]. Initial findings amongst cases of cocaine-associated agranulocytosis (CAA) sug-gest a similar association with HLA-B27 [2, 15].We performed a case–control study to confirm the association between CAA and HLA-B27 and used a novel SNP genotyping assay to identify genetic markers for CAA.MethodsSetting and study designWe conducted a case–control study of CAA using the British Columbia Centre for Disease Control’s (BCCDC) voluntary physician reporting system that communi-cated with emergency department staff and other health professionals through provincial medical health officers. Approval was obtained from the University of British Columbia Clinical Research Ethics Board.Study participantsCocaine-associated agranulocytosis cases, defined as individuals with a neutrophil count ≤0.5 per 109/L and laboratory confirmation or self-reported cocaine use at the time of diagnosis, were identified both retrospectively from 2008 and prospectively from case reports provided between January 2009 and October 2011. Case reports included patient demographics, clinical information, and past medical history [6, 19]. Eligible cases were recruited through study invitation letters distributed by the report-ing physician with an invitation to contact a research team member for enrollment. This study employed a 1:3 case–control ratio; controls were recruited using case-based snowball sampling (i.e. cases gave study information to people they knew to invite them to contact the research team if they were interested in participating in the study) and through a local harm reduction center. Controls were defined as individuals with no known history of agranu-locytosis and self-reported current cocaine use and matched on age (±5  years), sex and ethnicity including self-reported Aboriginal and Non-Aboriginal heritage. All participants were paid $25 to complete a survey question-naire and provide a saliva sample for genetic analysis.GenotypingGenomic DNA was purified from saliva samples using the QIAmp DNA purification system (Qiagen, Toronto, ON, Canada) according to the manufacturer’s protocol. Samples were genotyped using a previously described TaqMan-based assay specific for multiple variants of the HLA-B27 haplotype, which identifies the common HLA-B27 subtypes including HLA-B2702, B2705, B2706, B2707, and B2708 [20], followed by examination of two HLA-B27 tag SNPs (rs4349859 and rs13202464) using a multiplexed SNP genotyping assay with a custom Illu-mina iSelect genotyping panel (Illumina, San Diego, CA, USA). This custom panel tested 7,600 genetic vari-ations in 1,350 selected candidate genes involved in drug absorption, distribution, metabolism, and excretion (ADME) and also includes drug transporter and recep-tor variants. SNP genotype data were clustered manually using GenomeStudio software (Illumina, San Diego, CA, USA). The average sample call rate was 99.88% and all samples had call rates >99.6%. All SNPs described were in Hardy–Weinberg equilibrium.Statistical analysesBaseline characteristics were compared using the Fisher’s exact test for categorical variables and the unpaired t test for continuous variables. Genetic results were com-pared using the Fisher exact test for the dominant genetic model or the exact form of the Cochran–Armitage trend test for the additive genetic model.ResultsPatient characteristicsAs of October 2011, 51 individuals with one or more episodes of CAA were reported through the BCCDC voluntary physician reporting system. The average age was 40 years (range 22–64) and 55% were female. Ances-try information was self-reported; 51% reported Abo-riginal ancestry, 33% non-Aboriginal, 4% other and 12% unknown or missing. When asked about route of cocaine administration, 45% smoked only, 20% snorted only, 2% injected only, 8% used more than one route and 24% had missing information.Page 3 of 6Buxton et al. BMC Res Notes  (2015) 8:240 Of the 51 eligible CAA subjects, 37 did not partici-pate (predominantly due to inability to contact), 14 were enrolled, 6 were lost to follow-up (defined as initiated contact but either did not complete the questionnaire or did not provide a sample or both), and eight ultimately participated. The self-reported ancestry of the cases was predominantly Aboriginal (five); two cases identified as Caucasian and one as South Asian. Twenty-six controls were recruited and were similar to cases in terms of age, gender, ancestry (all eight non-Aboriginal were Cauca-sian), HIV and hepatitis C status and rheumatologic his-tory (Table 1).Although not systematically asked, two of the cases reported stopping cocaine use following their hospitali-zation for CAA.Association of CAA with HLA‑B27Taqman-based genotyping of the HLA-B27 haplotype revealed a significant association with CAA (Fisher exact p value = 0.017, odds ratio (OR) = 9.2, 95% confidence interval [CI], 1.5–54.6) (Table  2), replicating the pre-viously identified association of HLA-B27 with CAA. Overall, 62.5% of CAA cases carried the HLA-B27 allele, compared to 15.4% of the controls.Using a multiplexed SNP genotyping assay, an identi-cal association in complete linkage disequilibrium was observed between CAA and a 41 kb away HLA-B27 tag SNP rs4349859 (Fisher exact p value = 0.017, OR = 9.2 (95% CI 1.5–54.6) under a dominant genetic model and an additive genetic model (Fished exact p value 0.017) (Table  2). For a retrospective prediction of CAA, the TaqMan HLA-B27 test and rs4349859 SNP exhibited a sensitivity of 62.5% and specificity of 84.6%, and a posi-tive predictive value (PPV) of 71.4% and negative predic-tive value (NPV) of 88.0%.A second tag SNP, rs13202464, located 20 kb away and in LD with the tested HLA-B27 haplotype (Our cohort r2  =  0.55) and 21  kb away and in LD with rs4349859 (Our cohort r2 =  0.47; HapMap CEU D’ 1.0, r2 =  0.32) showed a significant, though slightly weaker association (Fisher exact p value = 0.042, OR = 6.8, 95% CI 1.1–41). Compared to the TaqMan HLA-B27 haplotype test and Table 1 Baseline characteristics of cases and controlsa Non aboriginal: cases 2 Caucasian, 1 South Asian; Controls all Caucasian.Characteristic Cases (N = 8) Controls (N = 26) P valueSex, no. (%) 0.681 Female 6 (75) 16 (62) Male 2 (25) 10 (38)Mean age, year (range) 37 (27–48) 39 (28–53) 0.617Self reported ancestry, no. (%) Aboriginal 5 (62) 18 (69) Non-aboriginala 3 (38) 8 (31)HIV positivity, no. (%) 1 (14) 4 (15) 1Hepatitis C positivity, no. (%) 1 (14) 12 (46) 0.116History of rheumatoid arthri-tis, no. (%)1 (14) 2 (8) 1History of unusual skin problems, no. (%)1 (14) 3 (12) 1Table 2 HLA-B27 variants associated with cocaine-associated agranulocytosisSNP single nucleotide polymorphism, CI confidence intervals.SNP haplotype Cases (N = 8) Controls (N = 26) Odds ratio (95% CI) P valueHLA-B27 9.2 (1.54–54.6) 0.017 +, no. (%) 5 (62.5) 4 (15.4) −, no. (%) 3 (37.5) 22 (84.6)rs4349859—dominant model 9.2 (1.54–54.6) 0.017 A_, no. (%) 5 (62.5) 4 (15.4) GG, no. (%) 3 (37.5) 22 (84.6)rs4349859—additive genetic model 0.017 AA, no. (%) 1 (12.5) 0 AG, no. (%) 4 (50) 4 (15.4) GG, no. (%) 3 (37.5) 22 (84.6)rs13202464—dominant model 6.8 (1.11–41.0) 0.042 G_, no. (%) 6 (75) 8 (30.8) AA, no. (%) 2 (25) 18 (69.2)rs13202464—additive genetic model 0.011 GG, no. (%) 2 (25) 0 AG, no. (%) 4 (50) 8 (30.8) AA, no. (%) 2 (25) 18 (69.2)Page 4 of 6Buxton et al. BMC Res Notes  (2015) 8:240 rs4349859, the rs13202464 variant exhibited an increased sensitivity (75.0%) with a reduced specificity (69.2%), reduced PPV (60.0%) and increased NPV (90.0%).Novel genetic variants associated with CAAIn the hypothesis-generating chip-based analysis of the 7,600 variants in the pharmacokinetic and phar-macodynamics SNP panel, a significant association was identified for a protective intergenic SNP near the ADRB1 (adrenoceptor beta 1) gene (exact Armitage p value =  9.1 ×  10(−6); p value =  0.046 after Bonferroni correction for multiple testing; estimated allelic odds ratio =  0.06 (95% CI 0.007–0.46) (Table 3). In addition, two other genetic variants exhibited strong trends (P value <0.001) but were not significantly associated after multiple testing correction in the ABCC12 (ATP bind-ing cassette subfamily C member 12, OR  =  10.0, 95% CI 2.7–36.8) and CYP11A1 (cytochrome P450 family 11 subfamily A polypetide 1, OR  =  7.4, 95% CI 2.1–26.6) genes (Table 3). The variant in ABCC12 is a non-synony-mous variant causing an Arg1117Cys amino acid change carried by 35% of the patients in this study, with rela-tively similar frequencies worldwide except for a higher frequency in some African populations. The intronic CYP11A1 variant was present in 75.0% of CAA cases, compared to 28.8% of controls. This variant exhibits rela-tively similar allele frequencies worldwide (27–49%).DiscussionAgranulocytosis is a severe adverse drug event emerg-ing as an important complication of crack cocaine use in Canada. Here, we report the first case–control study of the pharmacogenomic determinants of CAA. We demonstrate a significant replication of the association between CAA and HLA-B27 using both the standard TaqMan assay for HLA-B27 and two tag SNP markers previously associated with HLA-B27. A novel protective association was identified between CAA and an inter-genic variant near the ADRB1 gene, with adverse asso-ciations identified between CAA and variants in the ABCC12 and CYP11A1 genes. Our results confirm the genetic basis for the association between levamisole and CAA, a finding with important implications for identifying and managing at-risk individuals to prevent occurrence and relapse.HLA-B27 has been implicated in a wide range of pathology, including spondyloarthropathies; however, the genetic mechanism by which HLA-B27 acts in the auto-immune disease pathway remains largely undetermined [21]. One hypothesis, known as the linked gene theory, suggests that HLA-B27 is a marker for a nearby true sus-ceptibility locus [22], while other studies suggest it is not the sole mediator of pathology and its effects are modu-lated by other HLA and non-HLA genes [23]. Indeed, we observe a novel protective association between CAA and an intergenic variant near the ADRB1 gene, with adverse associations identified between CAA and variants in the ABCC12 and CYP11A1 genes. ADRB1 encodes the beta-1 adrenergic receptor, which when bound by catechola-mines stimulates a sympathetic nervous system response important in the regulation of cardiac function. Although levamisole contains neither a catechol nor an amine group, a single report in the literature has linked levami-sole to ADRB1 activity. Ogunbiyi et al. reported that in a bovine model, levamisole restored ADRB1 function—as measured by the productive of reactive oxygen species—in pulmonary alveolar macrophages in response to infec-tion with two distinct respiratory viruses [24]. Given the protective effect of the ADRB1 variant reported in the present study and this previous work reporting a benefi-cial immunomodulatory effect of levamisole in a phago-cytic cell population, this association between ADRB1 and CAA merits further investigation.Although the case–control study design supports the presence of an association, the sample population was small. Challenges were encountered in recruiting both cases and controls. As cases were identified through a voluntary physician reporting system, the busy clinical environment may have impeded enrollment. People with problematic substance use may discharge themselves against medical advice, before they were approached to participate in the study [25]. Moreover the target popu-lation was highly transient, with homelessness and lack of contact information following hospital discharge ham-pering follow-up efforts. Recruitment of controls was first attempted using case-based snowballing sampling, Table 3 Additional genetic variants associated with cocaine-associated agranulocytosisSNP single nucleotide polymorphism, CI confidence intervals, Chr chromosome.SNP Gene Chr Alleles Type Allele frequency (%)Cases Controls Odds ratio (95% CI) P value Corrected P valuers10885531 ADRB1 10 G/A Intragenic 0 53.8 0.06 (0.007–0.46) 9.09 × 10−6 0.043rs7193955 ABCC12 16 A/G Nonsynonymous 75 23.1 10 (2.7–36.8) 1.00 × 10−4 0.47rs2279357 CYP11A1 15 G/A Intronic 75 28.8 7.4 (2.1–26.6) 7.29 × 10−4 1Page 5 of 6Buxton et al. BMC Res Notes  (2015) 8:240 which ultimately produced few participants; therefore active recruitment was performed in a high-risk neigh-borhood. These issues are important considerations for future research in this vulnerable and at-risk population, particularly in situations where a timely response is war-ranted. Furthermore, our case definition included both confirmed and probable cases, as we were unable to con-firm the presence of levamisole in probable cases. It was assumed that persons who habitually used cocaine would likely be exposed to levamisole-tainted cocaine at some point during the study period due to the high preva-lence of this adulterant in circulating crack-cocaine [8]. However, since controls were also persons using crack-cocaine with undetermined levamisole presence, this exposure misclassification would be true for both groups and likely bias towards the null. Replication of the results using a larger sample and examining a dose–response relationship would further contribute to our understand-ing of CAA.Levamisole-induced agranulocytosis is an emerging clinical and public health concern among individuals using crack cocaine, and the potential complications of this adverse drug event are important considerations in the clinical care of this vulnerable population. A deeper understanding of CAA has allowed cases to be diagnosed and managed without invasive procedures such as a bone biopsy [6] and has facilitated efforts to inform people who use drugs about the importance of seeking medical assistance promptly when symptoms occur. In patients presenting with unexplained fever and agranulocytosis, clinicians are encouraged to consider the possibility of cocaine use. Recommended management of patients with CAA presenting with fever or infection includes support-ive care, and treatment with empiric intravenous broad spectrum antibiotics and granulocyte-colony stimulating factor (G-CSF or filgrastim) [6, 9].ConclusionAlthough our sample size was small the strength of the association confirms the primary hypothesis of a genetic association between CAA and HLA-B27. It is not practi-cal to test people who use cocaine for HLA-B27 status; however health care providers should warn people who are identified as having that that it is genetically deter-mined they are at high risk of reoccurrence if they con-tinue to use cocaine. As described by the health belief model, the perceived susceptibility and risk of recurrence of CAA, and the severity of the outcome and hospitaliza-tion may act as an internal cue to prompt health-promot-ing behavior such as stopping cocaine use or engaging in addiction treatment [26]. These genetic associations war-rant the attention of public health and knowledge transla-tion efforts to highlight the implications for susceptibility to this severe adverse drug event that may occur repeat-edly due to chronic cocaine use.Authors’ contributionsJB Conception and design, acquisition of data, revising the article critically. JO Interpretation of data, drafting the article. MK Acquisition of data, revising the article critically. CR Analysis and interpretation of data, revising the article critically. DT Acquisition of data, revising the article critically. RP Conception and design, acquisition of data, revising the article critically. JG Analysis and interpretation of data, revising the article critically. BC Analysis and interpreta-tion of data, revising the article critically. All authors read and approved the final manuscript.Author details1 BC Center for Disease Control, 655 West 12th Avenue, Vancouver, BC V5Z 4R4, Canada. 2 School of Population and Public Health, University of British Columbia, 2206 East Mall, Vancouver, BC V6T 1Z3, Canada. 3 Child and Family Research Institute, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada.  4 Drug and Poison Information Centre, BC Centre for Disease Control, 655 West 12th Avenue, Vancouver, BC V5Z 4R4, Canada. AcknowledgementsThe authors wish to thank all participants for their participation in the study, and health care providers and public health in British Columbia for reporting cases.This study was funded by the BC Centre for Disease Control. CR and BC receive support from The Canadian Institutes of Health Research and the Canadian Foundation for Innovation.Compliance with ethical guidelinesCompeting interestsThe authors declare no financial or non-financial competing interests.Received: 26 June 2014   Accepted: 1 June 2015References 1. Andersohn F, Konzen C, Garbe E (2007) Systematic review: agranulocyto-sis induced by nonchemotherapy drugs. Ann Intern Med 146(9):657–665 2. Czuchlewski DR, Brackney M, Ewers C, Manna J, Fekrazad MH, Martinez A et al (2010) Clinicopathologic features of agranulocytosis in the setting of levamisole-tainted cocaine. Am J Clin Pathol 133(3):466–472 3. Pisciotta AV (1990) Drug induced agranulocytosis peripheral destruction of polymorphonuclear leukocytes and their marrow precursors. Blood Rev 4:226–237 4. Thompson JS, Herbick JM, Klassen LW, Severson CD, Overlin VL, Blaschke JW et al (1980) Studies on levamisole-induced agranulocytosis. Blood 56(3):388–396 5. 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