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GSTP1 and TNF Gene Variants and Associations between Air Pollution and Incident Childhood Asthma: The… MacIntyre, Elaina A.; Brauer, Michael; Melén, Erik; Bauer, Carl Peter; Bauer, Mario; Berdel, Dietrich; Bergström, Anna; Brunekreef, Bert; Chan-Yeung, Moira; Klümper, Claudia; Fuertes, Elaine; Gehring, Ulrike; Gref, Anna; Heinrich, Joachim; Herbarth, Olf; Kerkhof, Marjan; Koppelman, Gerard H.; Kozyrskyj, Anita L.; Pershagen, Göran; Postma, Dirkje S.; Thiering, Elisabeth; Tiesler, Carla M.T.; Carlsten, Christopher; TAG, Study Group Apr 30, 2014

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418 volume 122 | number 4 | April 2014 • Environmental Health PerspectivesResearch | Children’s Health All EHP content is accessible to individuals with disabilities. A fully accessible (Section 508–compliant) HTML version of this article is available at http://dx.doi.org/10.1289/ehp.1307459. GSTP1 and TNF Gene Variants and Associations between Air Pollution and Incident Childhood Asthma: The Traffic, Asthma and Genetics (TAG) StudyElaina A. MacIntyre,1,2 Michael Brauer,1 Erik Melén,3,4 Carl Peter Bauer,5 Mario Bauer,6 Dietrich Berdel,7 Anna Bergström,3 Bert Brunekreef,8,9 Moira Chan-Yeung,10 Claudia Klümper,11 Elaine Fuertes,1,2 Ulrike Gehring,8 Anna Gref,3 Joachim Heinrich,2 Olf Herbarth,12 Marjan Kerkhof,13 Gerard H. Koppelman,14 Anita L. Kozyrskyj,15,16 Göran Pershagen,3 Dirkje S. Postma,17,18 Elisabeth Thiering,2 Carla M.T. Tiesler,2,19 and Christopher Carlsten,10 for the TAG Study Group1School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada; 2Institute of Epidemiology I, Helmholtz Zentrum München, German Research Centre for Environmental Health, Neuherberg, Germany; 3Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden; 4 Sachs’ Children’s Hospital, Stockholm, Sweden; 5Department of Pediatrics, Technical University of Munich, Munich, Germany; 6Department for Environmental Immunology, Helmholtz Centre for Environmental Research–UFZ, Leipzig, Germany; 7Department of Pediatrics, Marien-Hospital Wesel, Wesel, Germany; 8Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands; 9Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, the Netherlands; 10Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada; 11Leibniz Research Institute for Environmental Medicine, University of Düsseldorf, Düsseldorf, Germany; 12Environmental Medicine and Hygiene, University of Leipzig, Leipzig, Germany; 13Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands; 14Department of Pediatric Pulmonology and Pediatric Allergology, Beatrix Children’s Hospital, University of Groningen, University Medical Center Groningen, Groningen Research Institute for Asthma and COPD (GRIAC), Groningen, the Netherlands; 15Department of Pediatrics, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta, Canada; 16School of Public Health, University of Alberta, Edmonton, Alberta, Canada; 17University of Groningen, Department of Pulmonology, University Medical Center Groningen, Groningen, the Netherlands; 18GRIAC, Groningen, the Netherlands; 19Division of Metabolic Diseases and Nutritional Medicine, Ludwig-Maximilians-University of Munich, Dr. von Hauner Children’s Hospital, Munich, GermanyBackground: Genetics may partially explain observed heterogeneity in associations between traffic-related air pollution and incident asthma.oBjective: Our aim was to investigate the impact of gene variants associated with oxidative stress and inflammation on associations between air pollution and incident childhood asthma.Methods: Traffic-related air pollution, asthma, wheeze, gene variant, and potential confounder data were pooled across six birth cohorts. Parents reported physician-diagnosed asthma and wheeze from birth to 7–8 years of age (confirmed by pediatric allergist in two cohorts). Individual estimates of annual average air pollution [nitrogen dioxide (NO2), particulate matter ≤ 2.5 μm (PM2.5), PM2.5 absorbance, ozone] were assigned to each child’s birth address using land use regression, atmospheric modeling, and ambient monitoring data. Effect modification by variants in GSTP1 (rs1138272/Ala114Val and rs1695/IIe105Val) and TNF (rs1800629/G-308A) was investigated.results: Data on asthma, wheeze, potential confounders, at least one SNP of interest, and NO2 were available for 5,115 children. GSTP1 rs1138272 and TNF rs1800629 SNPs were associated with asthma and wheeze, respectively. In relation to air pollution exposure, children with one or more GSTP1 rs1138272 minor allele were at increased risk of current asthma [odds ratio (OR) = 2.59; 95% CI: 1.43, 4.68 per 10 μg/m3 NO2] and ever asthma (OR = 1.64; 95% CI: 1.06, 2.53) compared with homozygous major allele carriers (OR = 0.95; 95% CI: 0.68, 1.32 for current and OR = 1.20; 95% CI: 0.98, 1.48 for ever asthma; Bonferroni-corrected interaction p = 0.04 and 0.01, respectively). Similarly, for GSTP1 rs1695, associations between NO2 and current and ever asthma had ORs of 1.43 (95% CI: 1.03, 1.98) and 1.36 (95% CI: 1.08, 1.70), respectively, for minor allele carriers compared with ORs of 0.82 (95% CI: 0.52, 1.32) and 1.12 (95% CI: 0.84, 1.49) for homozygous major allele carriers (Bonferroni-corrected interaction p-values 0.48 and 0.09). There were no clear differences by TNF genotype.conclusions: Children carrying GSTP1 rs1138272 or rs1695 minor alleles may constitute a susceptible population at increased risk of asthma associated with air pollution.citation: MacIntyre EA, Brauer M, Melén E, Bauer CP, Bauer M, Berdel D, Bergström A, Brunekreef B, Chan-Yeung M, Klümper C, Fuertes E, Gehring U, Gref A, Heinrich J, Herbarth O, Kerkhof M, Koppelman GH, Kozyrskyj AL, Pershagen G, Postma DS, Thiering E, Tiesler CM, Carlsten C, TAG Study Group. 2014. GSTP1 and TNF gene variants and associations between air pol-lution and incident childhood asthma: the traffic, asthma and genetics (TAG) Study. Environ Health Perspect 122:418–424; http://dx.doi.org/10.1289/ehp.1307459IntroductionEvidence supporting an association between traffic-related air pollution and incident childhood asthma is growing although pub-lished estimates highlight variability across different populations. (Anderson et al. 2013; Gehring et al. 2010; Nordling et al. 2008). Genetic variation could be one expla-nation for this observed heterogeneity and gene–environment studies may help identify those children most susceptible to air pol-lution (Bråbäck and Forsberg 2009). Large studies that combine multiple birth cohorts are ideal for examining gene–environment interactions in the development of childhood asthma, particularly where there are similar assessments of air pollution and asthma across cohorts (London 2007).Genes related to oxidative stress are of spe-cial interest because of the biological mecha-nisms thought to underlie both the toxicity of traffic-related air pollution and the develop-ment of asthma (Kelly 2003). GSTP1 (the glutathione S-transferase pi gene) codes for an enzyme that metabolizes reactive oxygen species in the lung, and, despite considerable study, associations with atopic disease have not been consistent (Piacentini et al. 2013), pos-sibly because of additional host and environ-ment factors such as age (Salam et al. 2007). To date, GSTP1 polymorphisms have been reported to modify the association between traffic-related air pollution and allergic sensiti-zation (Melén et al. 2008), persistent wheeze (Schroer et al. 2009), and asthma (Carlsten Address correspondence to C. Carlsten, Vancouver General Hospital, 2775 Laurel St., 7th Floor, Vancouver, British Columbia, V5Z1M9 Canada. Telephone: (604) 875-4729. E-mail: carlsten@mail.ubc.caSupplemental Material is available online (http://dx.doi.org/10.1289/ehp.1307459).Initial discussions regarding the TAG collaboration took place at the AllerGen NCE workshop “Genes and the Environment: The Genesis of Asthma and Allergy” in 2009. We thank K. de Hoogh for providing the ozone exposure estimates from the APMoSPHERE project. We thank all children and parents for their cooperation. We also thank all technical and administrative support staff and the medical and fieldwork teams.Funding for the TAG study was provided by the AllerGen Network of Centers of Excellence. The BAMSE study was supported by the Swedish Research Council, the Swedish Research Council FORMAS (for Environment, Agricultural Sciences and Spatial Planning), the Swedish Heart–Lung Foundation, Stiftelsen Frimurare Barnhuset i Stockholm, the Stockholm County Council, the Swedish Environmental Protection Agency, and the Swedish Society for Medical Research. The PIAMA study is supported by the Netherlands Organization for Health Research and Development, the Netherlands Organization for Scientific Research, the Netherlands Asthma Fund, the Netherlands Ministry of Spatial Planning, Housing, and the Environment, and the Netherlands Ministry of Health, Welfare, and Sport. The GINIplus study was funded by Helmholtz Zentrum München and grants from the Federal Ministry for Education, Science, Research and Technology 292 (01 EE 94014). The LISAplus study was funded by Helmholtz Zentrum München, Munich Center of Health (MC Health) and partly by grants from the Federal Ministry for Education, Science, Research and Technology (01 EG 9705/2 and 01EG9732). Both GINIplus and LISAplus were co-funded by the German Network of Competency on Adiposity and the Federal Ministry for Environment (IUF, FZK 20462296). The CAPPS study was supported by the Canadian Institute of Health Research, the British Columbia Lung Association, and the Manitoba Medical Service Foundation. The SAGE study was supported by the Canadian Institute of Health Research. E.F. was sup-ported by the AllerGen NCE (Canadian Allergy and Immune Diseases Advanced Training Initiative) and the Canadian Institutes of Health Research (Sir Frederick Banting and Charles Best Canada Graduate Scholarship).The authors declare they have no actual or potential  competing financial interests.Received: 2 August 2013; Accepted: 24 January 2014; Advance Publication: 24 January 2014; Final Publication: 1 April 2014.Traffic, childhood asthma, and GSTP1Environmental Health Perspectives • volume 122 | number 4 | April 2014 419et al. 2011a) in individual birth cohorts; and in one study these strata-specific findings were further attenuated by TNF (tumor necrosis factor) polymorphisms (Melén et al. 2008). TNF mediates inflammation and is expressed in response to environmental pollutants (Wu et al. 2007). TNF polymorphisms have been associated with asthma (Aoki et al. 2006; Panasevich et al. 2010) and wheeze (Wu et al. 2007) and were previously reported to modify the association between ozone and asthma in healthy young adults (Yang et al. 2008).We initiated an international collabora-tion, the Traffic, Asthma and Genetics Study (TAG), to examine the influence of specific candidate genes on the relationship between traffic-related air pollution and the develop-ment of childhood asthma.MethodsThe Canadian Asthma Primary Prevention Study (CAPPS) is a randomized controlled study of high-risk children (Carlsten et al. 2011b). The Study of Asthma, Genes and the Environment (SAGE) is a Canadian population-based birth cohort (Kozyrskyj et al. 2009). The Children, Allergy, Milieu, Stockholm, Epidemiological Survey (BAMSE) is a population-based Swedish birth cohort (Melén et al. 2008). The German Infant Nutrition Intervention Study plus environ-mental and genetic influences on allergy (GINI) is a population-based birth cohort (Morgenstern et al. 2008). The Influence of Life-Style Factors on the Development of the Immune System and Allergies in East and West Germany plus the influence of traf-fic emissions and genetics (LISA) study is a population-based birth cohort (Morgenstern et al. 2008). The Prevention and Incidence of Asthma and Mite Allergy (PIAMA) study is a population-based birth cohort (Brunekreef et al. 2002). Detailed cohort descriptions are provided in the Supplemental Material, Appendix 1, and additional details on the TAG study have been previously published (MacIntyre et al. 2013). Parents participating in each cohort provided informed consent, and protocols were approved by all relevant ethical review boards.Physician-diagnosed asthma and wheeze symptoms were collected from parental ques-tionnaires. CAPPS and SAGE children were also evaluated by a pediatric allergist to con-firm asthma. Current asthma and current wheeze were ascertained at 8 years (6 years for GINI/LISA wheeze; 7 years for CAPPS asthma/wheeze). Ever asthma and ever wheeze were defined using every available  follow-up to 8 years. Children missing ≥ 2 follow-ups were excluded. Data were collected at slightly differ-ent time points, and there were minor differ-ences in question wording across the cohorts (MacIntyre et al. 2013).Annual average nitrogen dioxide (NO2) was estimated using traffic-related land use regression (LUR) models, except for the BAMSE cohort, which used dispersion mod-eling (Bellander et al. 2001). LUR models developed for PIAMA and LISA/GINI Munich (Germany) were based on data col-lected in 1999–2000 (Brauer et al. 2003), LISA/GINI Wesel (Germany) in 2003, LISA/GINI Leipzig (Germany) in 2004/2005 (Hochadel et al. 2006), CAPPS Vancouver (BC, Canada) in 2003 (Henderson et al. 2007), and SAGE and CAPPS Winnipeg (MB, Canada) in 2007 (Allen et al. 2010). For all study sites, integrated 14-day sampling campaigns were repeated over a 12-month period to capture seasonal patterns in air pol-lution. Protocols were specifically developed to spatially model long-term average pollut-ant concentrations. Potential predictors for traffic were screened based on their correla-tion with measured pollutants. Final mod-els were assessed based on the coefficient of determination and root mean square error from cross-validation. LUR estimated PM2.5 (particulate matter with diameter ≤ 2.5 μm) and PM2.5 absorbance (a measure of elemen-tal carbon estimated from filter reflectance measurements) were available for a subset of the combined cohort. For BAMSE, emis-sion data for traffic-generated nitrogen oxides (NOx) were collected for the years 1990 and 2000; and dispersion was estimated using a dilution model based on wind speed, direc-tion, and precipitation (Nordling et al. 2008). Additionally, ozone was estimated for the European cohorts using APMoSPHERE (Air Pollution Modelling for Support to Policy on Health and Environmental Risk in Europe) (Beelen et al. 2009), and for CAPPS/SAGE using inverse distance weighting of the closest three ambient monitors (within 50 km). NO2 was the only air pollution metric that was available across all six cohorts. PM2.5, PM2.5 absorbance, and ozone were used in sensi-tivity analyses because estimates of exposure to these pollutants were not available for all cohorts. Exposures were assigned for the first year of life based on geocoded birth addresses.Candidate genes were selected based on availability of data across all six cohorts. The following single-nucleotide polymorphisms (SNPs) were investigated: GSTP1 rs1138272 (merged with rs1799811; C→T: Ala114Val), GSTP1 rs1695 (merged with rs947894; A→G: Ile105Val), and TNF rs1800629 (G→A: –308). Genotypic analyses were conducted at sepa-rate facilities using a variety of methods: The Canadian birth cohorts both used the Illumina BeadArray system (Illumina, San Diego, CA, USA) following the manufacturer’s protocol [CAPPS: peripheral blood was collected at 7 years (Carlsten et al. 2011a); SAGE: buccal samples were collected at 9 years (Kozyrskyj et al. 2009)]. The PIAMA study collected buccal swabs or blood at 4–8 years and used Competitive Allele-Specific PCR (polymerase chain reaction) using KASParTM genotyping chemistry (KBiosciences, Hoddesdon, UK). (Koopman et al. 2002). The German studies and BAMSE used matrix-assisted laser desorp-tion/ionization–time of flight (MALDI-TOF) mass spectrometry (Sequenom, San Diego, CA, USA) following the manufacturer’s proto-col [GINI/LISA: blood samples were collected at 6–10 years; BAMSE: blood samples were collected at 4 years (Melén et al. 2008)]. The German studies additionally used the restric-tion fragment length polymorphism approach for GSTP1 rs1695 (Slama et al. 2010) and high-resolution melting-PCR using LightSNiP hybridization probe for GSTP1 rs1138272 (TIB MolBiol, Berlin, Germany).Data transfer and harmonization occurred in several stages. First, each cohort sent data dictionaries (translated to English, where applicable) to the main study center at the University of British Columbia. From each of the data dictionaries, a list of key variables was compiled and sent to each cohort to request data extraction. Data extracts were then pre-pared by each cohort and sent to the main study center for harmonization and merging. Data were collected at different time points across the cohorts, with differences in ques-tionnaire wording and case definitions [see MacIntyre et al. (2013) for additional infor-mation]. In the pooled data set, new vari-ables for this analysis were derived from data  common to all cohorts.Statistical analysis. We examined the main effect estimate for each SNP using dominant genetic models. Effect modifica-tion was examined in adjusted analyses of NO2 (per 10-μg/m3 increase) and asthma/wheeze, stratified by genotype. Interactions between genotype and NO2 were investi-gated using the log likelihood ratio for mod-els with and without product interaction terms. Models were adjusted for potential confounders available across each cohort: study, city, infant sex, birth weight (< 2,500, 2,500–4,000, > 4,000 g), parental history of allergy (yes/no), maternal age at birth (≤ 24, 25–29, 30–34, ≥ 35 years), maternal smoking reported anytime during pregnancy (yes/no), environmental tobacco smoke reported in the home (yes/no), and intervention (yes/no). All pooled analyses used weighted logistic regres-sion to adjust for inclusion of case–control data (Richardson et al. 2007). Sensitivity analyses investigated additional exposure metrics available for only a subset of pooled data [PM2.5 (per 4-μg/m3 increase), PM2.5 absorbance (per 0.5 10–5/m increase), and ozone (per 10-μg/m3 increase)). All p-values were corrected for multiple testing using Bonferroni adjustment, and adjusted p-values MacIntyre et al.420 volume 122 | number 4 | April 2014 • Environmental Health Perspectives< 0.05 were considered statistically signifi-cant. All analyses used SAS version 9.2 (SAS Institute Inc., Cary, NC, USA).ResultsTable 1 describes the population character-istics for children included in at least one analyses (Ntotal = 5,115). The population used for TAG had a lower prevalence of low birth weight (< 2,500 g) babies (3% vs. 12%), fewer mothers < 25 years of age (6% vs. 9%), more parents with reported atopy (55% vs. 47%), and fewer mothers who smoked during pregnancy (14% vs. 17%), compared to the total recruited population for each cohort.A total of 5,115 children had complete data on asthma, wheeze, potential confound-ers, NO2, and at least one SNP (4,596 with GSTP1 rs1138272, 4,748 with GSTP1 rs1695, 4,483 with TNF rs1800629); 22% were part of an intervention, 52% were male, 29% were exposed to environmental tobacco smoke in the home, 55% had parental his-tory of atopy, and 19% were born to moth-ers ≥ 35 years of age. Parents of 7.5% of the children reported current asthma (ranging from 2.3% to 40.5% in individual cohorts), 18% reported ever asthma (4.6–41.6%), 12.5% reported current wheeze (7.0–69.0%), 45% ever wheeze (35.2–69.0%), and 7.6% reported both ever asthma and current wheeze (“active asthma”; 2.8–46.6%).Table 2 describes pollutant distributions for NO2, PM2.5, PM2.5 absorbance, and ozone. Data are presented for the pooled data set and by cohort or combinations of cohorts in which an identical LUR model was used in two different cohorts (Munich, Wesel, and Winnipeg). In general, the mean NO2 con-centrations for Germany and the Netherlands were similar, whereas those for one of the Canadian locations (Winnipeg) and Sweden were lower. For the 2,743 children in sensitiv-ity analyses, NO2 was correlated with PM2.5 absorbance (r = 0.72), weakly correlated with PM2.5 (r = 0.23), and inversely correlated with ozone (r = –0.19). The inverse correla-tion between NO2 and ozone was attributable to the atmospheric chemistry unique to each pollutant; and the weak correlation between PM2.5 and PM2.5 absorbance was likely attributable to differences in the composition and sources of air pollution in each region.The population distribution and number of asthma cases by genotype are presented in Table 3. All SNPs satisfied Hardy–Weinberg equilibrium based on chi-square statistics (p < 0.05).Main effect estimates. Table 4 shows the main effect estimates for associations of the genotypes and air pollution exposures with the respiratory outcomes based on the pooled data for all cohorts combined. Effect estimates were calculated for homozygous and hetero-zygous minor allele carriers versus homo-zygous major allele carriers. The adjusted Table 1. Population characteristics for the pooled TAG data set and for each individual cohort [n (%)].Characteristic Pooled BAMSE CAPPS GINI LISA SAGE PIAMATotal population 5,115 (100) 912 (100) 346 (100) 1,126 (100) 697 (100) 178 (100) 1,856 (100)Intervention 1,114 (21.8) NA 181 (52.3) 528 (46.9) NA NA 405 (21.8)Male sex 2,661 (52.0) 484 (53.1) 189 (54.6) 584 (51.9) 361 (51.8) 96 (53.9) 947 (51.0)Birth weight (g)< 2,500  150 (2.9) 45 (4.9) 16 (4.6) 19 (1.7) 0 (–) 13 (7.3) 57 (3.1)2,500–4,000  4,085 (79.9) 706 (77.4) 256 (74.0) 952 (84.6) 587 (84.2) 132 (74.2) 1,452 (78.2)> 4,000  880 (17.2) 161 (17.6) 74 (21.4) 155 (13.8) 110 (15.8) 33 (18.5) 347 (18.7)Maternal smoking during pregnancy 688 (14.1) 119 (13.1) 29 (8.4) 149 (15.7) 91 (13.6) 18 (10.2) 282 (15.3)Environmental tobacco smoke at homea 1,465 (28.6) 164 (18.0) 71 (20.5) 315 (28.0) 158 (22.7) 34 (19.1) 723 (39.0)Parental atopy 2,802 (54.8) 530 (58.1) 319 (92.2) 459 (40.8) 348 (49.9) 126 (70.8) 1,020 (55.0)Mother’s age at birth (years)≤ 24 285 (5.6) 73 (8.0) 23 (6.7) 44 (3.9) 38 (5.5) 22 (12.4) 85 (4.5)25–29 1,574 (30.8) 287 (31.5) 86 (24.9) 314 (27.9) 221 (31.7) 54 (30.3) 612 (33.0)30–34 2,278 (44.5) 363 (40.2) 125 (36.1) 553 (49.1) 306 (44.1) 74 (41.6) 857 (46.2)≥ 35 978 (19.1) 189 (20.7) 112 (32.4) 215 (19.1) 132 (18.9) 28 (15.7) 302 (16.3)Current asthma 381 (7.5) 112 (12.3) 62 (17.9) 42 (3.7) 16 (2.3) 72 (40.5) 77 (4.2)Ever asthma 878 (17.7) 272 (29.8) 144 (41.6) 86 (8.2) 30 (4.6) 72 (40.5) 274 (15.0)Current wheeze 623 (12.5) 162 (17.9) 76 (22.0) 112 (10.1) 63 (9.4) 80 (69.0) 130 (7.0)Ever wheeze 2,243 (44.9) 535 (58.7) 159 (46.0) 383 (35.2) 278 (40.0) 80 (69.0) 808 (44.0)Ever asthma AND current wheeze 372 (7.6) 122 (13.5) 61 (17.3) 41 (3.9) 18 (2.8) 54 (46.6) 76 (4.2)NA, not applicable.aDefined as one or more person in the household regularly smoking indoors during the first year of life (exceptions: second year of life in GINI; eighth year of life in SAGE). Table 2. Annual average pollutant statistics for the first year of life for pooled data and for each individual model.Statistic Pooled data Sweden (BAMSE)Munich, Germany (LISA/GINI)Wesel and Leipzig, Germany (LISA/GINI)The Netherlands (PIAMA)Vancouver, Canada (CAPPS)Winnipeg, Canada (CAPPS/SAGE)NO2 (μg/m3)Mean ± SD 22.7 ± 8.1 16.7 ± 9.1 27.5 ± 6.3 22.9 ± 3.5 24.6 ± 7.4 32.5 ± 6.0 12.2 ± 3.6IQR 10.3 13.8 8.1 4.9 11.2 7.1 5.2Range 2.2–66.8 2.2–49.9 19.5–66.9 13.9–41.4 12.6–58.4 18.9–55.1 4.1–21.5Ozone (μg/m3)Mean ± SD 36.8 ± 6.5 NA 43.9 ± 2.1 39.4 ± 3.6 33.8 ± 4.7 21.2 ± 2.8 NAIQR 9.1 NA 1.0 6.9 4.3 4.8 NARange 13.3–55.9 NA 38.8–55.9 32.3–48.6 13.3–47.3 13.4–27.0 NAPM2.5 (μg/m3)Mean ± SD 15.2 ± 3.4 NA 13.3 ± 1.3 NA 16.7 ± 1.9 5.6 ± 2.6 NAIQR 4.1 NA 1.5 NA 3.4 3.9 NARange 0.0–25.1 NA 11.9–21.9 NA 13.5–25.2 0.0–10.0 NAPM2.5 absorbance (10–5/m)Mean ± SD 1.7 ± 0.4 NA 1.7 ± 0.3 1.6 ± 0.2 1.7 ± 0.4 1.6 ± 1.2 NAIQR 0.4 NA 0.3 0.2 0.6 1.1 NARange 0.0–5.0 NA 1.4–4.3 0.8–2.3 0.8–3.7 0.0–5.0 NAAbbreviations: IQR, interquartile range; NA, not applicable.Traffic, childhood asthma, and GSTP1Environmental Health Perspectives • volume 122 | number 4 | April 2014 421odds ratio (OR) for GSTP1 rs1138272 and current asthma was 1.57 (95% CI: 1.16, 2.14; Bonferroni-corrected p-value = 0.012). Findings were similar for GSTP1 rs1138272 and ever asthma (OR = 1.49; 95% CI: 1.20, 1.84; corrected p-value < 0.001) and current wheeze plus ever asthma (OR = 1.61; 95% CI: 1.22, 2.14; corrected p-value = 0.003). The adjusted OR for GSTP1 rs1695 and cur-rent asthma was 0.87 (95% CI: 0.68, 1.12) in pooled analysis; associations between rs1695 and remaining respiratory outcomes were consistent with the null hypothesis. The adjusted OR for TNF rs1800629 and current asthma was 1.20 (95% CI: 0.92, 1.57). The association between ever wheeze and TNF rs1800629 was not statistically sig-nificant after adjusting for multiple testing (OR = 1.18; 95% CI: 1.02, 1.37; corrected p-value = 0.087). Statistically significant asso-ciations were estimated for NO2 and ever asthma (OR = 1.23; 95% CI: 1.03–1.46 per 10-μg/m3 increase; corrected p-value = 0.019) and for PM2.5 and current asthma (OR = 2.08; 95% CI: 1.35–3.19 per 4-μg/m3 increase; corrected p-value = 0.001).Genotype stratification and interaction. The associations between NO2 during the first year of life and respiratory outcomes, stratified by genotype, and interaction p-values are presented in Table 5 for pooled data. Effect estimates were elevated and statis-tically significant in carriers of minor alleles of GSTP1 rs1138272 and rs1695 in models for current asthma (corrected p-value for minor allele carriers = 0.006; corrected p-value for homozygous major allele carriers = 0.766; corrected p-values for interaction = 0.039) and ever asthma (corrected p-value for minor allele carriers = 0.048; corrected p-value for homozygous major allele carriers = 0.432; corrected p-values for interaction = 0.087), respectively. Overall, effect estimates were greater for minor allele carriers than for their respective homozygous major allele carriers in models for current asthma and ever asthma. Findings for TNF generally supported the null hypothesis.For children who were minor allele car-riers for both GSTP1 SNPs (rs1138272 and rs1695; Ntotal = 728) there were statistically significant associations between NO2 and current asthma (OR = 2.37; 95% CI: 1.31, Table 3. Population distribution by minor allele carriers and homozygous major allele carriers for GSTP1 and TNF (pooled data).SNP genotype No. of childrenCurrent asthma cases [n (%)]Children in the highest tertile  of NO2 (n)Children with current asthma in the highest tertile of NO2 [n (%)]GSTP1 rs1138272TT/TC 789 80 (10.1) 224 15 (6.7)CC 3,807 287 (7.5) 1,144 57 (5.0)GSTP1 rs1695GG/GA 2,740 199 (7.3) 873 34 (3.9)AA 2,008 170 (8.5) 618 39 (6.3)TNF rs1800629AA/AG 1,411 119 (8.4) 421 31 (7.4)GG 3,072 244 (7.9) 917 40 (4.4)Table 4. Main genetic and environmental effect estimates for GSTP1, TNF, and NO2 for asthma and wheeze at school age (pooled data).SNP, exposureCurrent asthma Ever asthma Current wheeze Ever wheeze Ever asthma and current wheezen aORa (95% CI) pb n aORa (95% CI) pb n aORa (95% CI) pb n aORa (95% CI) pb n aORa (95% CI) pbGSTP1 rs1138272 TT/TC v. CC 4,596 1.57 (1.16, 2.14)0.012 4,465 1.49 (1.20, 1.84)< 0.001 4,483 1.15 (0.84, 1.57)0.560 4,487 1.01 (0.84, 1.22)0.918 4,377 1.61 (1.22, 2.14) 0.003GSTP1 rs1695 GG/GA v. AA 4,748 0.87 (0.68, 1.12)0.285 4,635 0.91 (0.77, 1.08)0.430 4,659 1.02 (0.81, 1.29)0.860 4,633 0.96 (0.83, 1.10)0.803 4,547 0.94 (0.75, 1.19) 0.628TNF rs1800629 AA/AG v. GG 4,483 1.20 (0.92, 1.57)0.258 4,356 1.04 (0.87, 1.26)0.647 4,371 1.22 (0.95, 1.56)0.345 4,373 1.18 (1.02, 1.37)0.087 4,267 1.10 (0.85, 1.41) 0.628Traffic-related NO2  (per 10-μg/m3 increase)5,115 1.14 (0.98, 1.48)0.322 4,965 1.23 (1.03, 1.46)0.019 4,999 1.12 (0.89, 1.40)0.337 4,995 1.04 (0.91, 1.18)0.610 4,874 1.13 (0.87, 1.46) 0.374Ozone (per 10-μg/m3 increase) 2,743 0.81 (0.51, 1.27)0.351 2,671 0.86 (0.66, 1.13)0.277 2,728 1.29 (0.88, 1.88)0.197 2,704 0.90 (0.74, 1.10)0.318 2,659 1.01 (0.62, 1.67) 0.960Traffic-related PM2.5  (per 4–μg/m3 increase)2,743 2.08 (1.35, 3.19)0.001 2,671 1.17 (0.86, 1.60)0.323 2,728 1.33 (0.90, 1.95)0.147 2,704 0.95 (0.74, 1.22)0.687 2,659 1.46 (0.93, 2.32) 0.102Traffic-related PM2.5 absorbance (per 0.5 10–5/m increase)2,743 1.07 (0.91, 1.26)0.441 2,671 1.06 (0.95, 1.19)0.283 2,728 1.06 (0.93, 1.21)0.395 2,704 1.00 (0.91, 1.10)0.963 2,659 1.07 (0.92, 1.25) 0.369aOR, adjusted odds ratio.aAdjusted for study, city, intervention, infant sex, maternal age at birth, maternal smoking during pregnancy, environmental tobacco smoke in the home, birth weight, and parental atopy. bp-Values were corrected for multiple testing using the Bonferroni method.Table 5. Association between traffic-related NO2 during the first year of life and asthma and wheeze at school age (pooled data).GeneCurrent asthma Ever asthma Current wheeze Ever wheeze Ever asthma and current wheezen aORa (95% CI) pb n aORa (95% CI) pb n aORa (95% CI) pb n aORa (95% CI) pb n aORa (95% CI) pbGSTP1 rs1138272TT/TC 789 2.59 (1.43, 4.68) 0.006 769 1.64 (1.06, 2.53) 0.075 772 1.26 (0.71, 2.23) 0.821 775 1.17 (0.82, 1.65) 0.467 757 1.30 (0.67, 2.52) 0.669CC 3,807 0.95 (0.68, 1.32) 0.766 3,696 1.20 (0.98, 1.48) 0.140 3,711 1.09 (0.82, 1.45) 0.821 3,712 1.09 (0.92, 1.29) 0.467 3,620 1.17 (0.85, 1.61) 0.669Interactionc 0.039 0.012 0.096 0.024 0.180GSTP1 rs1695GG/GA 2,704 1.43 (1.03, 1.98) 0.096 2,677 1.36 (1.08, 1.70) 0.048 2,695 1.18 (0.86, 1.60) 0.821 2,692 1.11 (0.93, 1.33) 0.467 2,632 1.16 (0.82, 1.65) 0.669AA 2,008 0.82 (0.52, 1.32) 0.626 1,958 1.12 (0.84, 1.49) 0.432 1,964 1.03 (0.72, 1.46) 0.888 1,951 0.98 (0.79, 1.23) 0.886 1,915 1.09 (0.70, 1.68) 0.709Interactionc 0.484 0.087 0.187 0.026 0.436TNF rs1800629AA/AG 1,411 1.30 (0.85, 2.00) 0.448 1,372 1.29 (0.94, 1.78) 0.143 1,383 1.04 (0.68, 1.59) 0.888 1,384 1.14 (0.86, 1.49) 0.467 1,350 1.13 (0.70, 1.82) 0.709GG 3,072 1.07 (0.72, 1.57) 0.766 2,984 1.21 (0.97, 1.52) 0.140 2,988 1.24 (0.92, 1.68) 0.821 2,989 1.09 (0.91, 1.30) 0.467 2,917 1.15 (0.81, 1.63) 0.669Interactionc 0.210 0.345 0.096 0.337 0.436aOR, adjusted odds ratio.aFor a 10-μg/m3 increase in NO2. Adjusted for study, city, intervention, infant sex, maternal age at birth, maternal smoking during pregnancy, environmental tobacco smoke in the home, birth weight, and parental atopy. bp-Values were corrected for multiple testing using the Bonferroni method. cp-Values for interaction were calculated based on the likelihood-ratio tests between models with and without interaction terms and were corrected for multiple testing using the Bonferroni method. MacIntyre et al.422 volume 122 | number 4 | April 2014 • Environmental Health Perspectives4.34 per 10 μg/m3 NO2; p = 0.005) and ever asthma (OR = 1.57; 95% CI: 1.04, 2.37; p = 0.032); analyses in remaining GSTP1 rs1138272*rs1695 strata (rs1138272 TT/TC and rs1695 AA; rs1138272 CC and rs1695 GG/GA; rs1138272 CC and rs1695 AA) were nonsignificant (data not shown).Sensitivity analyses. Sensitivity analyses are presented in the Supplemental Material, Tables S1–S12. The following cohorts (or cohort subsets) had assigned ozone, PM2.5, and PM2.5 absorbance; CAPPS Vancouver, LISA, GINI, and PIAMA. A total of 2,743 children had complete data on asthma, wheeze, potential confounders, NO2, ozone, PM2.5, PM2.5 absorbance, and at least one SNP of interest (n = 2,367 with GSTP1 rs1138272; n = 2,559 with GSTP1 rs1695; n = 2,318 with TNF rs1800629). Pooled estimates were recalculated for these addi-tional air pollution metrics (see Supplemental Material, Tables S1–S3, respectively). In analyses stratified by genotype, there was an association between ozone and current wheeze (OR = 3.67; 95% CI: 1.05, 12.7; Ntotal = 405) in GSTP1 rs1138272 minor allele carriers, and between PM2.5 and cur-rent asthma in all strata examined, although small sample size for some strata resulted in highly imprecise estimates. The results of multipollutant models examining NO2 and PM2.5, stratified by genotype, are presented in Supplemental Material, Table S4. NO2 effect estimates were sensitive to inclusion of PM2.5 in some analyses, but these results should be interpreted cautiously because data were  available for only four cohorts.The analyses applied to the pooled data were also applied to each individual cohort (see Supplemental Material, Tables S5–S10). Results showed some heterogeneity in the magnitude of effect estimate, but findings were generally not statistically significant due to small sample sizes. For example, the odds of current asthma per 10 μg/m3 NO2 for GSTP1 rs1138272 minor allele carriers were 2.19 (0.54, 8.86) in BAMSE, 6.33 (95% CI: 0.66, 60.8) in GINI and LISA Munich, 5.44 (95% CI: 1.23, 24.0) in GINI and LISA Wesel, 2.27 (95% CI: 0.69, 7.49) in PIAMA, 2.30 (95% CI: 0.02, 256) in CAPPS Vancouver, and 19.20 (95% CI: 0.79, 466) in CAPPS and SAGE Winnipeg, versus 2.59 (95% CI: 1.43, 4.68) in pooled data. Further, the interactions between NO2 and genotype in pooled analyses were robust in reanalyses that sequentially excluded individual cohorts (data not shown).Finally, analyses were rerun after restricting the pooled cohort to only those children in an observational (nonintervention) study arm (Ntotal = 3,695; see Supplemental Material, Table S11) and to children of Caucasian race (Ntotal = 4,821; see Supplemental Material, Table S12; data available only for CAPPS and SAGE). Results of these final sensitivities were in general agreement with findings in the full data set.DiscussionThis study is the first Canadian–European consortium to examine the interaction between traffic-related air pollution and can-didate genes of oxidative stress and inflam-mation in the development of childhood asthma. The pooled data set included 381 children with current asthma and 878 with ever asthma, and provided an unprecedented opportunity to identify populations geneti-cally at risk for asthma development in rela-tion to traffic-related air pollution. Our findings suggest that children with variant GSTP1 genotypes may constitute a suscep-tible population at increased risk of asthma associated with air pollution.The purpose of this work was to estimate the effect of two oxidative stress and inflam-mation genes on the relationship between traffic-related air pollution and the develop-ment of asthma in childhood. Asthma was associated with GSTP1 rs1138272 in the study population. In analyses of NO2 and respiratory outcome, stratified by genotype, minor allele carriers for GSTP1 rs1138272 and GSTP1 rs1695 appeared to be more sus-ceptible to the harmful effects of air pollution than their homozygous major allele–carry-ing counterparts. The interaction between GSTP1 rs1138272 and NO2 was statistically significant in models for current asthma, ever asthma, and ever wheeze.In this study we used data from six birth cohorts. Five of these cohorts have previ-ously reported associations between traffic-related air pollution and respiratory outcomes during childhood (Carlsten et al. 2011b; Gehring et al. 2010; Morgenstern et al. 2008; Nordling et al. 2008); and three have pre-viously reported gene–air pollution interac-tions in the development of childhood asthma (Carlsten et al. 2011a; Melén et al. 2008; Nordling et al. 2008). The BAMSE cohort reported a statistically significant association between NOx and asthma at 4 years of age in children who were both minor allele carriers for GSTP1 rs1695 and homozygous major allele carriers for TNF rs1800629 (OR = 2.9; 95% CI: 1.0–8.2 for a 44-μg/m3 increase in NOx) (Melén et al. 2008). We did not find strong evidence for a gene–gene–environment interaction in the pooled data set, although effect estimates were similar to those reported by Melén et al. (2008). The CAPPS study, which was particularly limited by sample size and did not have any homozygous minor allele carriers for GSTP1 rs1138272, reported a negative but not statistically significant asso-ciation between NO2 and asthma at 7 years of age in children who were heterozygous for GSTP1 rs1138272 (OR = 0.76; 95% CI: 0.18–3.30 for a 7.2-μg/m3 increase in NO2; NTotal = 25) (Carlsten et al. 2011a). Finally, the PIAMA study has previously reported effect modification by interaction analyses for multiple TLR2 and TLR4 SNPs in the association between air pollution (using the same LUR models we presented) and child-hood asthma in a subset of the PIAMA cohort (NTotal = 916; Kerkhof et al. 2010); but the current study is the first examination of GSTP1 and TNF.Polymorphisms in genes related to oxi-dative stress (NQO1, GSTM1, and GSTP1) have been associated with increased risk of asthma. Previous work has reported reduc-tions in GSTP1 expression among rs1138272 and rs1695 minor allele carriers (Moyer et al. 2008). Minor allele carriers of rs1695 were found to be at increased risk for asthma in numerous different study populations (Tamer et al. 2004), particularly when children were exposed to environmental tobacco smoke (Palmer et al. 2006) or living in regions with high air pollution (Romieu et al. 2006); and impaired lung function was observed in schoolchildren living in areas of high urban air pollution (Reddy et al. 2012). It has also been suggested that GST genes may modify the lung response after co-exposure to die-sel exhaust and allergen (Gilliland 2009). Conversely, the rs1695 SNP has been neg-atively associated with asthma (Aynacioglu et al. 2004; Fryer et al. 2000) and was associ-ated with a lower risk of incident asthma with exercise in communities with high (vs. low) levels of ozone (Islam et al. 2009). Few studies have had the opportunity to evaluate GSTP1 rs1138272 and air pollution, partly because of the low frequency of minor allele carriers.The inflammatory response gene TNF (rs1800629) has been reported to modify the relationship between ozone and asthma (Yang et al. 2008) and indoor home dampness and asthma (Tsai et al. 2011); however, similar to our results, no effect modification was noted in the association between traffic-related NO2 and adult-onset asthma (Castro-Giner et al. 2009).In the present study we pooled data across six cohorts; but unfortunately, of the many SNPs associated with oxidative stress and inflammation, only three were typed across all cohorts. It is possible that this study was underpowered; however, homozygous and heterozygous minor allele carriers were combined into one category for all analy-ses (Melén et al. 2008; Salam et al. 2007), and three of the TAG cohorts intentionally increased the proportion of children with asthma or wheeze through study design.NO2 is especially useful as a marker of within-city variability in exposure to Traffic, childhood asthma, and GSTP1Environmental Health Perspectives • volume 122 | number 4 | April 2014 423traffic-related pollution and has been used as a marker of traffic pollution exposure in previous epidemiological investigations (Brunekreef 2007; Clark et al. 2010; Emenius et al. 2003; Gehring et al. 2010). Our results were not fully replicated in the subset (n = 2,743) of children with available data on additional markers of traffic-related (PM2.5 and PM2.5 absorbance) and regional (ozone) air pollution. The air pollution metrics exam-ined here were considered only as markers for traffic-related air pollution; and the goal of this work was not to highlight the importance of one metric over another, but instead to highlight the significant potential impact of this air pollution source.The TAG cohorts are unique in that they have individually assigned exposures with high spatial resolution for the first year of life based on residential address at birth—thus capturing the important exposure window during early life (Clark et al. 2010; Gehring et al. 2010). Although measurements for the LUR models were not originally taken during the first year of life, detailed investigations, including two conducted in our study areas, found the spatial distribution of traffic-related pollutants to be stable for up to 12 years (Eeftens et al. 2011; Gulliver et al. 2013; Wang et al. 2012). LUR model estimates were temporally adjusted using fixed-site monitoring data collected during the actual first year of life for cohorts that completed NO2 monitoring campaigns > 8 years after the birth year (CAPPS and SAGE).An additional strength of the cohorts we included is that each recruited pregnant women or newborns and was therefore able to assess the development of asthma from birth. Ascertainment of physician-diagnosed asthma and wheeze symptoms via parental questionnaire is reasonably accurate (Burr 1992), particularly in countries with universal health care, where lack of insurance does not hinder access to health care. Further, the two cohorts with the highest prevalence of asthma (SAGE and CAPPS) also completed exam-inations by a pediatric allergist to confirm parent-reported asthma diagnoses. Further, although there were slight differences in ques-tionnaire wording for asthma and predictors for air pollutant models, the general consis-tency in effect estimate direction and mag-nitude across analyses stratified by cohort or land use regression model (see Supplemental Material, Tables S5–S10) is reassuring and further  supports our findings.The findings presented here may have been confounded by exposures associated with oxidative stress or airway inflammation. We adjusted for environmental tobacco smoke and parental history of asthma or atopic dis-ease, but it was not possible to adjust pooled analyses for the presence of allergens in the home or ethnicity. Smoking was associated with increased odds of disease in all pooled analyses; however, this finding was statisti-cally significant only for “ever asthma” (data not shown). Sensitivity analyses excluding non-Caucasian children from the Canadian cohorts (< 80% Caucasian) were completed to ensure that our findings were not influ-enced by the ethnic composition of our population (Spielman et al. 2007); the propor-tion of Caucasian children in the European cohorts was very high (98%) and they were not restricted in the sensitivity. Finally, addi-tional sensitivity analyses excluding children assigned to an intervention (Supplemental Material, Table S11; CAPPS, GINI, and PIAMA) were completed to ensure that our findings were not affected by preventive mea-sures; this is particularly important because the CAPPS study has reported a protective effect (for children assigned to the intervention) on the development of childhood asthma up to 7 years of age (Chan-Yeung et al. 2000).Our findings add to previous research by providing further insight into the complex relationship between genetics and environ-mental factors in the development of child-hood asthma. 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