Open Collections

Li, Boaz; Leung, Samuel; Elishaev, Maria; Cheng, Wan Hei; Mocci, Giuseppe; Björkegren, Johan L. M.; Lai, Chi; Singh, Amrit; Wang, Ying

Multidisciplinary Digital Publishing Institute

Diffuse intimal thickening (DIT) is a pre-clinical stage of atherosclerosis characterized by thickened intima. The molecular basis of its susceptibility to atherogenesis is unknown, and mechanistic investigations cannot be performed in commonly used mouse models, in which DIT does not exist. Vascular smooth muscle cells (SMCs) are the predominant cell type that occupies the intima and media of DIT. The molecular differences between these two layers may reveal the earliest phenotypic changes in SMCs to promote atherosclerosis. We benchmarked the RNA quality of human coronary arteries from autopsies (n = 7) and explanted hearts (n = 7) and performed Visium spatial gene expression on tissue sections with DIT. Although autopsy samples met the RNA quality standard for Visium (DV200 ≥ 30%), only arteries from explanted hearts exhibited reliable sequencing performance. Genes enriched in TGF-β-mediated remodeling of the extracellular matrix were overrepresented in the intima. SMCs enriched in the intima are dedifferentiated, but unlike those in the atherosclerotic lesions, they are not pro-inflammatory. Our findings indicate that autopsy samples are not ideal to distinguish subtle differences among cell phenotypes. SMCs in thickened intima may lead to lipid retention but not necessarily the onset of atherosclerosis.

Article

eng

Vancouver : University of British Columbia Library

10.14288/1.0448721

Medicine, Faculty of; Anesthesiology, Pharmacology and Therapeutics, Department of; Pathology and Laboratory Medicine, Department of

10.3390/ijms26051949

Faculty; Researcher

DSpace