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The Microbiological Burden of Short-Term Catheter Reuse in Individuals with Spinal Cord Injury: A Prospective Study Miller, Tiev; Lange, Dirk (Microbiologist); Kizhakkedathu, Jayachandran N.; Yu, Kai; Felix, Demian; Samejima, Soshi; Shackleton, Claire; Malik, Raza N; Sachdeva, Rahul; Walter, Matthias; Krassioukov, Andrei V.

Abstract

Despite the risk of developing catheter-associated urinary tract infections (CAUTI), catheter reuse is common among people with spinal cord injury (SCI). This study examined the microbiological burden and catheter surface changes associated with short-term reuse. Ten individuals with chronic SCI reused their catheters over 3 days. Urine and catheter swab cultures were collected daily for analysis. Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) analyses were used to assess catheter surface changes. Catheter swab cultures showed no growth after 48 h (47.8%), skin flora (28.9%), mixed flora (17.8%), or bacterial growth (5.5%). Asymptomatic bacteriuria was found for most participants at baseline (n = 9) and all at follow-up (n = 10). Urine samples contained Escherichia coli (58%), Klebsiella pneumoniae (30%), Enterococcus faecalis (26%), Acinetobacter calcoaceticus–baumannii (10%), Pseudomonas aeruginosa (6%) or Proteus vulgaris (2%). Most urine cultures showed resistance to one or more antibiotics (62%). SEM images demonstrated structural damage, biofilm and/or bacteria on all reused catheter surfaces. XPS analyses also confirmed the deposition of bacterial biofilm on reused catheters. Catheter surface changes and the presence of antibiotic-resistant bacteria were evident following short-term reuse, which may increase susceptibility to CAUTI in individuals with SCI despite asymptomatic bacteriuria.

Item Metadata

Title
The Microbiological Burden of Short-Term Catheter Reuse in Individuals with Spinal Cord Injury: A Prospective Study
Creator
Miller, Tiev; Lange, Dirk (Microbiologist); Kizhakkedathu, Jayachandran N.; Yu, Kai; Felix, Demian; Samejima, Soshi; Shackleton, Claire; Malik, Raza N; Sachdeva, Rahul; Walter, Matthias; Krassioukov, Andrei V.
Contributor
International Collaboration on Repair Discoveries; Stone Centre at Vancouver General Hospital; University of British Columbia. Centre for Blood Research; University of British Columbia. Life Sciences Institute; G.F. Strong Rehabilitation Centre; Vancouver Coastal Health Authority
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2023-07-07
Description
Despite the risk of developing catheter-associated urinary tract infections (CAUTI), catheter reuse is common among people with spinal cord injury (SCI). This study examined the microbiological burden and catheter surface changes associated with short-term reuse. Ten individuals with chronic SCI reused their catheters over 3 days. Urine and catheter swab cultures were collected daily for analysis. Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) analyses were used to assess catheter surface changes. Catheter swab cultures showed no growth after 48 h (47.8%), skin flora (28.9%), mixed flora (17.8%), or bacterial growth (5.5%). Asymptomatic bacteriuria was found for most participants at baseline (n = 9) and all at follow-up (n = 10). Urine samples contained Escherichia coli (58%), Klebsiella pneumoniae (30%), Enterococcus faecalis (26%), Acinetobacter calcoaceticus–baumannii (10%), Pseudomonas aeruginosa (6%) or Proteus vulgaris (2%). Most urine cultures showed resistance to one or more antibiotics (62%). SEM images demonstrated structural damage, biofilm and/or bacteria on all reused catheter surfaces. XPS analyses also confirmed the deposition of bacterial biofilm on reused catheters. Catheter surface changes and the presence of antibiotic-resistant bacteria were evident following short-term reuse, which may increase susceptibility to CAUTI in individuals with SCI despite asymptomatic bacteriuria.
Subject
intermittent urethral catheterization; urinary tract infections; bacteria; asymptomatic colonization; electron microscopy; photoelectron spectroscopy; lower urinary tract symptoms; neurogenic urinary bladder disorder; spinal cord injury
Genre
Article
Type
Text
Language
eng
Date Available
2023-08-01
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0434637
URI
http://hdl.handle.net/2429/85359
Affiliation
Medicine, Faculty of; Non UBC; Biomedical Engineering, School of; Occupational Science and Occupational Therapy, Department of; Pathology and Laboratory Medicine, Department of; Physical Therapy, Department of; Urologic Sciences, Department of
Citation
Biomedicines 11 (7): 1929 (2023)
Publisher DOI
10.3390/biomedicines11071929
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher
Rights URI
https://creativecommons.org/licenses/by/4.0/
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CC BY 4.0