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IL-4Rα blockade reduces influenza-associated morbidity in a murine model of allergic asthma Shahangian, Kimia; Ngan, David A.; Chen, H. H. Rachel; Oh, Yeni; Tam, Anthony; Wen, Jing; Cheung, Chung; Knight, Darryl; Dorscheid, Delbert R.; Hackett, Tillie-Louise; Hughes, Michael R.; McNagny, Kelly M.; Hirota, Jeremy A.; Niikura, Masahiro; Man, S. F. P.; Sin, Don D.

Abstract

Background: Asthma was identified as the most common comorbidity in hospitalized patients during the 2009 H1N1 influenza pandemic. We determined using a murine model of allergic asthma whether these mice experienced increased morbidity from pandemic H1N1 (pH1N1) viral infection and whether blockade of interleukin-4 receptor α (IL-4Rα), a critical mediator of Th2 signalling, improved their outcomes. Methods: Male BALB/c mice were intranasally sensitized with house dust mite antigen (Der p 1) for 2 weeks; the mice were then inoculated intranasally with a single dose of pandemic H1N1 (pH1N1). The mice were administered intraperitoneally anti-IL-4Rα through either a prophylactic or a therapeutic treatment strategy. Results: Infection with pH1N1 of mice sensitized to house dust mite (HDM) led to a 24% loss in weight by day 7 of infection (versus 14% in non-sensitized mice; p 

Item Metadata

Title
IL-4Rα blockade reduces influenza-associated morbidity in a murine model of allergic asthma
Creator
Shahangian, Kimia; Ngan, David A.; Chen, H. H. Rachel; Oh, Yeni; Tam, Anthony; Wen, Jing; Cheung, Chung; Knight, Darryl; Dorscheid, Delbert R.; Hackett, Tillie-Louise; Hughes, Michael R.; McNagny, Kelly M.; Hirota, Jeremy A.; Niikura, Masahiro; Man, S. F. P.; Sin, Don D.
Contributor
University of British Columbia. Centre for Heart Lung Innovation; St. Paul's Hospital (Vancouver, B.C.); University of British Columbia. Biomedical Research Centre
Publisher
BioMed Central
Date Issued
2021-03-02
Description
Background: Asthma was identified as the most common comorbidity in hospitalized patients during the 2009 H1N1 influenza pandemic. We determined using a murine model of allergic asthma whether these mice experienced increased morbidity from pandemic H1N1 (pH1N1) viral infection and whether blockade of interleukin-4 receptor α (IL-4Rα), a critical mediator of Th2 signalling, improved their outcomes. Methods: Male BALB/c mice were intranasally sensitized with house dust mite antigen (Der p 1) for 2 weeks; the mice were then inoculated intranasally with a single dose of pandemic H1N1 (pH1N1). The mice were administered intraperitoneally anti-IL-4Rα through either a prophylactic or a therapeutic treatment strategy. Results: Infection with pH1N1 of mice sensitized to house dust mite (HDM) led to a 24% loss in weight by day 7 of infection (versus 14% in non-sensitized mice; p 
Genre
Article
Type
Text
Language
eng
Date Available
2021-03-03
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0396029
URI
http://hdl.handle.net/2429/77431
Affiliation
Medicine, Faculty of; Other UBC; Non UBC; Medicine, Department of
Citation
Respiratory Research. 2021 Mar 02;22(1):75
Publisher DOI
10.1186/s12931-021-01669-0
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
The Author(s)
Rights URI
http://creativecommons.org/licenses/by/4.0/
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Attribution 4.0 International (CC BY 4.0)