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The aging lung: tissue telomere shortening in health and disease Everaerts, Stephanie; Lammertyn, Elise J.; Martens, Dries S.; De Sadeleer, Laurens J.; Maes, Karen; van Batenburg, Aernoud A.; Goldschmeding, Roel; van Moorsel, Coline H. M.; Dupont, Lieven J.; Wuyts, Wim A.; Vos, Robin; Gayan-Ramirez, Ghislaine; Kaminski, Naftali; Hogg, James C.; Janssens, Wim; Verleden, Geert M.; Nawrot, Tim S.; Verleden, Stijn E.; McDonough, John E.; Vanaudenaerde, Bart M.

Abstract

Background: Telomere shortening has been associated with several lung diseases. However, telomere length is generally measured in peripheral blood leucocytes rather than in lung tissue, where disease occurs. Consequently, telomere dynamics have not been established for the normal human lung nor for diseased lung tissue. We hypothesized an age- and disease-dependent shortening of lung tissue telomeres. Methods: At time of (re-)transplantation or autopsy, 70 explant lungs were collected: from unused donors (normal, n = 13) and patients with cystic fibrosis (CF, n = 12), chronic obstructive pulmonary disease (COPD, n = 11), chronic hypersensitivity pneumonitis (cHP, n = 9), bronchiolitis obliterans syndrome (BOS) after prior transplantation (n = 11) and restrictive allograft syndrome (RAS) after prior transplantation (n = 14). Lungs were inflated, frozen and then scanned using CT. Four tissue cores from distinct lung regions were sampled for analysis. Disease severity was evaluated using CT and micro CT imaging. DNA was extracted from the samples and average relative telomere length (RTL) was determined using real-time qPCR. Results: The normal lungs showed a decrease in RTL with age (p 

Item Metadata

Title
The aging lung: tissue telomere shortening in health and disease
Creator
Everaerts, Stephanie; Lammertyn, Elise J.; Martens, Dries S.; De Sadeleer, Laurens J.; Maes, Karen; van Batenburg, Aernoud A.; Goldschmeding, Roel; van Moorsel, Coline H. M.; Dupont, Lieven J.; Wuyts, Wim A.; Vos, Robin; Gayan-Ramirez, Ghislaine; Kaminski, Naftali; Hogg, James C.; Janssens, Wim; Verleden, Geert M.; Nawrot, Tim S.; Verleden, Stijn E.; McDonough, John E.; Vanaudenaerde, Bart M.
Contributor
James Hogg iCAPTURE Centre (University of British Columbia); St. Paul's Hospital (Vancouver, B.C.)
Publisher
BioMed Central
Date Issued
2018-05-11
Description
Background: Telomere shortening has been associated with several lung diseases. However, telomere length is generally measured in peripheral blood leucocytes rather than in lung tissue, where disease occurs. Consequently, telomere dynamics have not been established for the normal human lung nor for diseased lung tissue. We hypothesized an age- and disease-dependent shortening of lung tissue telomeres. Methods: At time of (re-)transplantation or autopsy, 70 explant lungs were collected: from unused donors (normal, n = 13) and patients with cystic fibrosis (CF, n = 12), chronic obstructive pulmonary disease (COPD, n = 11), chronic hypersensitivity pneumonitis (cHP, n = 9), bronchiolitis obliterans syndrome (BOS) after prior transplantation (n = 11) and restrictive allograft syndrome (RAS) after prior transplantation (n = 14). Lungs were inflated, frozen and then scanned using CT. Four tissue cores from distinct lung regions were sampled for analysis. Disease severity was evaluated using CT and micro CT imaging. DNA was extracted from the samples and average relative telomere length (RTL) was determined using real-time qPCR. Results: The normal lungs showed a decrease in RTL with age (p 
Subject
Cystic fibrosis; Chronic obstructive pulmonary disease; Chronic hypersensitivity pneumonitis; Chronic lung allograft dysfunction; BOS; RAS; Cellular senescence; Telomere length
Genre
Article
Type
Text
Language
eng
Date Available
2018-05-14
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0366284
URI
http://hdl.handle.net/2429/65852
Affiliation
Other UBC; Non UBC
Citation
Respiratory Research. 2018 May 11;19(1):95
Publisher DOI
10.1186/s12931-018-0794-z
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
The Author(s).
Rights URI
http://creativecommons.org/licenses/by/4.0/
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Attribution 4.0 International (CC BY 4.0)