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Improving the stability of 11C–labeled L-methionine with ascorbate Woods, Michael; Leung, Leo; Frantzen, Kari; Garrick, Jennifer G; Zhang, Zhengxing; Zhang, Chengcheng; English, Wade; Wilson, Don; Bénard, François; Lin, Kuo-Shyan

Abstract

Background: Carbon-11 labeled L-methionine (11C–MET) is a popular tracer used in the clinic for imaging brain tumors with positron emission tomography. However, the stability of 11C–MET in its final formulation is not well documented in literature. Recently, we observed fast degradation of HPLC-purified 11C–MET over time, and systematic investigation was conducted to identify the cause. Results: In this study, we verified the degraded product as 11C–labeled methionine sulfoxide (11C–METSO). To minimize oxidation, ascorbate (100 ppm) was added to the HPLC eluant, and the resulting HPLC-purified 11C–MET was stable in the final formulation solution without noticeable degradation for up to 1 h after the end of synthesis. Conclusions: Our data suggest that to minimize degradation, ascorbate can be added to the 11C–MET formulation solution especially if it is not administered into patients soon after the end of synthesis.

Item Metadata

Title
Improving the stability of 11C–labeled L-methionine with ascorbate
Creator
Woods, Michael; Leung, Leo; Frantzen, Kari; Garrick, Jennifer G; Zhang, Zhengxing; Zhang, Chengcheng; English, Wade; Wilson, Don; Bénard, François; Lin, Kuo-Shyan
Publisher
Springer International Publishing
Date Issued
2017-10-04
Description
Background: Carbon-11 labeled L-methionine (11C–MET) is a popular tracer used in the clinic for imaging brain tumors with positron emission tomography. However, the stability of 11C–MET in its final formulation is not well documented in literature. Recently, we observed fast degradation of HPLC-purified 11C–MET over time, and systematic investigation was conducted to identify the cause. Results: In this study, we verified the degraded product as 11C–labeled methionine sulfoxide (11C–METSO). To minimize oxidation, ascorbate (100 ppm) was added to the HPLC eluant, and the resulting HPLC-purified 11C–MET was stable in the final formulation solution without noticeable degradation for up to 1 h after the end of synthesis. Conclusions: Our data suggest that to minimize degradation, ascorbate can be added to the 11C–MET formulation solution especially if it is not administered into patients soon after the end of synthesis.
Subject
C-11 L-methionine; Stability; Homocysteine, Methionine sulfoxide; Oxidation; Ascorbate; Positron emission tomography
Genre
Article
Type
Text
Language
eng
Date Available
2018-06-11
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0368615
URI
http://hdl.handle.net/2429/66284
Affiliation
Medicine, Faculty of; Non UBC; Radiology, Department of
Citation
EJNMMI Radiopharmacy and Chemistry. 2017 Oct 04;2(1):13
Publisher DOI
10.1186/s41181-017-0032-x
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
The Author(s)
Rights URI
http://creativecommons.org/licenses/by/4.0/
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Attribution 4.0 International (CC BY 4.0)