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Sphingosine-1-phosphate induces COX-2 expression and PGE2 production in human granulosa cells through a S1P₁/₃-mediated YAP signaling Cheng, Jung-Chien; Chang, Hsun-Ming; Liu, Pang-Pin; Leung, P. C. K.
Abstract
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that can regulate various physiological and pathological processes. The expression of S1P has been detected in human follicular fluid. In addition, two S1P receptors, S1P₁ and S1P₃, are expressed at a high level in human granulosa cells. Cyclooxygenase-2 (COX-2)-derived prostaglandin E2 (PGE2) production plays a critical role in the regulation of ovulation. However, thus far, the effect of S1P on COX-2 expression and PGE2 production in human granulosa cells remains unknown. In the present study, our results demonstrated that treatment with S1P significantly induced COX-2, but not COX-1, expression and increased PGE2 production in human granulosa cells. The stimulatory effects of S1P on COX-2 expression and PGE2 production were attenuated by treatment with specific antagonist of S1P₁ or S1P₃ and siRNA-mediated knockdown of S1P₁ or S1P₃. In addition, the COX-2 expression was induced by S1P₁ or S1P₃ agonist treatment. Interestingly, treatment with S1P activated YAP signaling via S1P₁ and S1P₃. Moreover, knockdown of YAP partially attenuated S1P-induced COX-2 expression and PGE2 production. These results provide evidence that S1P induces COX-2 expression and PGE2 production in human granulosa cells through a S1P₁/₃-mediated YAP signaling pathway.
Item Metadata
Title |
Sphingosine-1-phosphate induces COX-2 expression and PGE2 production in human granulosa cells through a S1P₁/₃-mediated YAP signaling
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Alternate Title |
S1P induces COX-2 expression through YAP
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Creator | |
Contributor | |
Date Issued |
2016-06
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Description |
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that can regulate various physiological and pathological processes. The expression of S1P has been detected in human follicular fluid. In addition, two S1P receptors, S1P₁ and S1P₃, are expressed at a high level in human granulosa cells. Cyclooxygenase-2 (COX-2)-derived prostaglandin E2 (PGE2) production plays a critical role in the regulation of ovulation. However, thus far, the effect of S1P on COX-2 expression and PGE2 production in human granulosa cells remains unknown. In the present study, our results demonstrated that treatment with S1P significantly induced COX-2, but not COX-1, expression and increased PGE2 production in human granulosa cells. The stimulatory effects of S1P on COX-2 expression and PGE2 production were attenuated by treatment with specific antagonist of S1P₁ or S1P₃ and siRNA-mediated knockdown of S1P₁ or S1P₃. In addition, the COX-2 expression was induced by S1P₁ or S1P₃ agonist treatment. Interestingly, treatment with S1P activated YAP signaling via S1P₁ and S1P₃. Moreover, knockdown of YAP partially attenuated S1P-induced COX-2 expression and PGE2 production. These results provide evidence that S1P induces COX-2 expression and PGE2 production in human granulosa cells through a S1P₁/₃-mediated YAP signaling pathway.
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Subject | |
Genre | |
Type | |
Language |
eng
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Date Available |
2017-06-01
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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DOI |
10.14288/1.0318721
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URI | |
Affiliation | |
Citation |
Cheng J, Chang H, Liu P, Leung PCK. Sphingosine-1-phosphate induces COX-2 expression and PGE2 production in human granulosa cells through a S1P1/3-mediated YAP signaling. Cellular Signalling. 2016;28(6):643-651.
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Publisher DOI |
10.1016/j.cellsig.2016.03.006
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Peer Review Status |
Reviewed
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Scholarly Level |
Faculty; Postdoctoral; Graduate; Unknown
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Rights URI | |
Aggregated Source Repository |
DSpace
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Rights
Attribution-NonCommercial-NoDerivatives 4.0 International