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Tissue engineered models to probe cell-microenvironmental interactions in Cancer and Regeneration

Banff International Research Station for Mathematical Innovation and Discovery

The interface between a tumour and the adjacent stroma is a site of great importance for tumour development. At this site, carcinoma cells are highly proliferative, undergo invasive phenotypic changes, and directly interact with surrounding stromal cells, such as cancer-associated fibroblasts (CAFs) and immune cells, which further exert pro-tumourigenic effects. Here we describe the development of two tissue engineered platforms to probe these interactions: GLAnCE (Gels for Live Analysis of Compartmentalized Environments), an easy-to-use hydrogel-culture platform for investigating CAF-tumour cell interactions in vitro at a tumour-stroma interface, and TRACER a scaffold-based strategy that enables isolation of cells from specific regions within the tumour microenvironment to probe how cell-cell interactions and functions vary across gradients of microenvironmental factors such as oxygen.

Mathematics; Biology and other natural sciences; Partial differential equations; Mathematical biology

video/mp4

Author affiliation: University of Toronto

2021-01-16

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/77110

Unreviewed

http://creativecommons.org/licenses/by-nc-nd/4.0/