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Role of DNA Topology in Biological Machines and Evolutionarily Related Biological Functions: Chemical Chirality in Site-Specific DNA Recombination Jayaram, Makkuni
Description
<p class=MsoNormal style='text-align:justify'><b style='mso-bidi-font-weight: normal'><u><span lang=EN-US style='font-family:Arial'>Difference topology</span></u></b><span lang=EN-US style='font-family:Arial'>: The DNA path within a high-order DNA-protein assembly can be revealed from the topology of DNA knots and links formed by the action of <span class=SpellE>Flp</span> or <span class=SpellE>Cre</span> site-specific recombinase on target sites placed close to (and flanking) the assembly. This â analysis is experimentally simple, and has broad applicability. <b style='mso-bidi-font-weight:normal'><u>Choice between alternative reaction mechanisms</u></b>: Recombination product topologies resulting from a fixed synaptic topology can help distinguish between alternative modes of site arrangement and strand exchange by a given recombinase enzyme. <b style='mso-bidi-font-weight:normal'><u>Conserved biological functions revealed by conserved topology of DNA loci in vivo</u></b>:<span style="mso-spacerun:yes"> </span>DNA topology can reveal evolutionary relationships between two diverged DNA loci that perform analogous but distinct biological functions. The unusual positive chromatin writhe at the centromeres of yeast chromosomes is shared by the partitioning locus of an extrachromosomal circular DNA element present in the yeast nucleus. This finding lends credence to the possible origin of these loci from a common ancestor that once directed the segregation of both the chromosomes and the plasmid during cell division. <b style='mso-bidi-font-weight:normal'><u>Chemical chirality in DNA recombination</u></b>: The active site of the <span class=SpellE>Flp</span>/<span class=SpellE>Cre</span> recombinase contains two conserved positively charged side-chains (<span class=SpellE>Arg</span>-I and <span class=SpellE>Arg</span>-II) responsible for compensating the negative charge on the non-<span class=SpellE>brridging</span> oxygen atoms of the scissile phosphate group. When one of the oxygens is replaced by a neutral group, one of the arginine residues becomes dispensable. The reactivity of DNA substrates containing substitutions at either of the two oxygens with <span class=SpellE>Flp</span>/<span class=SpellE>Cre</span> lacking either of the two <span class=SpellE>arginines</span> reveals the chirality of <span class=SpellE>Arg</span>-oxygen interactions, and defines the <span class=SpellE>stereochemical</span> course of the recombination reaction. <o:p></o:p></span></p>
Item Metadata
Title |
Role of DNA Topology in Biological Machines and Evolutionarily Related Biological Functions: Chemical Chirality in Site-Specific DNA Recombination
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Creator | |
Publisher |
Banff International Research Station for Mathematical Innovation and Discovery
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Date Issued |
2019-03-27T09:01
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Description |
<p class=MsoNormal style='text-align:justify'><b style='mso-bidi-font-weight:
normal'><u><span lang=EN-US style='font-family:Arial'>Difference topology</span></u></b><span
lang=EN-US style='font-family:Arial'>: The DNA path within a high-order
DNA-protein assembly can be revealed from the topology of DNA knots and links
formed by the action of <span class=SpellE>Flp</span> or <span class=SpellE>Cre</span>
site-specific recombinase on target sites placed close to (and flanking) the
assembly. This â analysis is experimentally simple, and has broad applicability.
<b style='mso-bidi-font-weight:normal'><u>Choice between alternative reaction
mechanisms</u></b>: Recombination product topologies resulting from a fixed
synaptic topology can help distinguish between alternative modes of site
arrangement and strand exchange by a given recombinase enzyme. <b
style='mso-bidi-font-weight:normal'><u>Conserved biological functions revealed
by conserved topology of DNA loci in vivo</u></b>:<span
style="mso-spacerun:yes"> </span>DNA topology can reveal evolutionary
relationships between two diverged DNA loci that perform analogous but distinct
biological functions. The unusual positive chromatin writhe at the centromeres
of yeast chromosomes is shared by the partitioning locus of an extrachromosomal
circular DNA element present in the yeast nucleus. This finding lends credence
to the possible origin of these loci from a common ancestor that once directed
the segregation of both the chromosomes and the plasmid during cell division. <b
style='mso-bidi-font-weight:normal'><u>Chemical chirality in DNA recombination</u></b>:
The active site of the <span class=SpellE>Flp</span>/<span class=SpellE>Cre</span>
recombinase contains two conserved positively charged side-chains (<span
class=SpellE>Arg</span>-I and <span class=SpellE>Arg</span>-II) responsible for
compensating the negative charge on the non-<span class=SpellE>brridging</span>
oxygen atoms of the scissile phosphate group. When one of the oxygens is
replaced by a neutral group, one of the arginine residues becomes dispensable.
The reactivity of DNA substrates containing substitutions at either of the two
oxygens with <span class=SpellE>Flp</span>/<span class=SpellE>Cre</span>
lacking either of the two <span class=SpellE>arginines</span> reveals the
chirality of <span class=SpellE>Arg</span>-oxygen interactions, and defines the
<span class=SpellE>stereochemical</span> course of the recombination reaction. <o:p></o:p></span></p>
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Extent |
29.0 minutes
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Subject | |
Type | |
File Format |
video/mp4
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Language |
eng
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Notes |
Author affiliation: University of Texas at Austin
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Series | |
Date Available |
2019-09-24
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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DOI |
10.14288/1.0380967
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URI | |
Affiliation | |
Peer Review Status |
Unreviewed
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Scholarly Level |
Faculty
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Rights URI | |
Aggregated Source Repository |
DSpace
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Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International