Title	DNA unlinking by Xer recombination
Creator	Vazquez, Mariel
Publisher	Banff International Research Station for Mathematical Innovation and Discovery
Date Issued	2013-11-21
Description	Newly replicated circular chromosomes are topologically linked. XerCD-dif- FtsK recombination acts in the replication termination region of the Escherichia coli chromosome to remove links introduced during homologous recombination and replication, whereas Topoisomerase IV removes replication links only. Based on gel mobility patterns of the products of recombination, a stepwise unlinking pathway has been proposed. We show definitively that there is a unique shortest pathway of unlinking by XerCD-dif-FtsK that strictly reduces the complexity of the links at every step. We delineate the mechanism of action of the enzymes at each step along this pathway and provide a 3D interpretation of the results. This is joint work with Koya Shimokawa, Kai Ishihara, Ian Grainge and David Sherratt.
Extent	41 minutes
Subject	Mathematics; Biology and other natural sciences; Manifolds and cell complexes; Mathematical biology
Type	Moving Image
File Format	video/mp4
Language	eng
Notes	Author affiliation: Mathematics Department, San Francisco State University
Series	BIRS Workshop Lecture Videos (Banff, Alta)
Date Available	2014-08-07
Provider	Vancouver : University of British Columbia Library
Rights	Attribution-NonCommercial-NoDerivs 2.5 Canada
DOI	10.14288/1.0043789
URI	http://hdl.handle.net/2429/49741
Affiliation	Non UBC
Peer Review Status	Unreviewed
Scholarly Level	Faculty
Rights URI	http://creativecommons.org/licenses/by-nc-nd/2.5/ca/
Aggregated Source Repository	DSpace

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DNA unlinking by Xer recombination Vazquez, Mariel

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