- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Assessment of peripheral blood components as predictive...
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Assessment of peripheral blood components as predictive biomarkers of radiation-induced lung injury in four mouse models Popescu, Sandra
Abstract
Purpose: Thoracic radiotherapy may lead to significant injury to nor- mal lung tissue. Herein, this thesis modelled the onset of distress following high-dose thoracic radiation in two congenic mouse strains with varying post-irradiation survival time and assessed blood traits for a predictor of radiation-induced lung injury. Methods: Pneum1 and Radpf1 mice were given 14 Gy to the whole thorax in-house using the RS2000 small animal irradiation unit (225 kVp; 1.625 Gy/min), or remained as controls. Irradiated and control mice were monitored weekly and euthanized 1 week post irradiation, 16 weeks post irradiation and at experimental end. Blood was collected at time of sacrifice for a Complete Blood Count and Mammalian Comprehensive Chemistry tests. Additional to in-house data, blood data for C57BL/6 and C3H/HeJ mice was surveyed from the Mouse Phenome Database to determine a correlation between blood traits in four mouse strains and their known survival time following lung irradiation. Results: Female Pneum1 and Radpf1 mice differed in survival time following 14 Gy thoracic radiation. In these strains, only serum globulin and sodium measured in control mice of 24-25 weeks of age differed significantly by strain (p<0.0001 and p=0.0004) and correlated with survival with marginal significance (p=0.09 and p=0.07). Neutrophil and lymphocyte differentials measured in control C57BL/6, C3H/HeJ, Pneum1, and Radpf1 mice of 8 -10 weeks of age significantly differed by strain (p<0.0001) and correlated with survival with marginal significance (p=0.08 and p=0.06). Conclusion: Blood traits assessed in this thesis were not found to significantly correlate with survival following thoracic radiation. No predictive biomarkers of radiation-induced lung injury were found.
Item Metadata
| Title |
Assessment of peripheral blood components as predictive biomarkers of radiation-induced lung injury in four mouse models
|
| Creator | |
| Supervisor | |
| Publisher |
University of British Columbia
|
| Date Issued |
2023
|
| Description |
Purpose: Thoracic radiotherapy may lead to significant injury to nor- mal lung tissue. Herein, this thesis modelled the onset of distress following high-dose thoracic radiation in two congenic mouse strains with varying post-irradiation survival time and assessed blood traits for a predictor of radiation-induced lung injury.
Methods: Pneum1 and Radpf1 mice were given 14 Gy to the whole thorax in-house using the RS2000 small animal irradiation unit (225 kVp; 1.625 Gy/min), or remained as controls. Irradiated and control mice were monitored weekly and euthanized 1 week post irradiation, 16 weeks post irradiation and at experimental end. Blood was collected at time of sacrifice for a Complete Blood Count and Mammalian Comprehensive Chemistry tests. Additional to in-house data, blood data for C57BL/6 and C3H/HeJ mice was surveyed from the Mouse Phenome Database to determine a correlation between blood traits in four mouse strains and their known survival time following lung irradiation.
Results: Female Pneum1 and Radpf1 mice differed in survival time following 14 Gy thoracic radiation. In these strains, only serum globulin and sodium measured in control mice of 24-25 weeks of age differed significantly by strain (p<0.0001 and p=0.0004) and correlated with survival with marginal significance (p=0.09 and p=0.07). Neutrophil and lymphocyte differentials measured in control C57BL/6, C3H/HeJ, Pneum1, and Radpf1 mice of 8 -10 weeks of age significantly differed by strain (p<0.0001) and correlated with survival with marginal significance (p=0.08 and p=0.06).
Conclusion: Blood traits assessed in this thesis were not found to significantly correlate with survival following thoracic radiation. No predictive biomarkers of radiation-induced lung injury were found.
|
| Genre | |
| Type | |
| Language |
eng
|
| Date Available |
2023-11-23
|
| Provider |
Vancouver : University of British Columbia Library
|
| Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
|
| DOI |
10.14288/1.0437876
|
| URI | |
| Degree | |
| Program | |
| Affiliation | |
| Degree Grantor |
University of British Columbia
|
| Graduation Date |
2024-02
|
| Campus | |
| Scholarly Level |
Graduate
|
| Rights URI | |
| Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International